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Unique Features of Human Protein Arginine Methyltransferase 9 (PRMT9) and its Substrate RNA Splicing Factor SF3B2.

Hadjikyriacou A, Yang Y, Espejo A, Bedford MT, Clarke SG
J Biol Chem. 2015 May 15. pii: jbc.M115.659433. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
67607GST-PRMT9 WTbacterial expression of wild type human PRMT9
67608GST-PRMT9 L182A/D183A/I184A/G185Abacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutant
67609GST-PRMT9 D258G mutantbacterial expression of GST-PRMT9 D258G Double E loop mutant
67610GST-PRMT9 G260E mutantbacterial expression of GST-PRMT9 G260E Double E loop mutant
67611GST-PRMT9 deltaN (aa 21-350)bacterial expression of GST-PRMT9 missing residues 21-350
67612GST-PRMT9 deltaC 350 (stop)bacterial expression of GST-PRMT9 missing residues 351-845
67614GST-PRMT9 deltaTPR mutantbacterial expression of GST-PRMT9 missing residues 21-139
67615GST-SF3B2 wild type aa 401-550 fragmentbacterial expression of wild type GST-SF3B2 fragment (401-550)
79674GST-SF3B2 R508K aa 401-550 fragmentbacterial expression of GST-SF3B2 fragment (401-550) with R508L methylation site mutation
79675GFP-PRMT9mammalian expression of GFP-PRMT9
79676GFP-PRMT9 L182A/D183A/I184A/G185Amammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

Antibodies from Article