GST-PRMT9 D258G mutant
(Plasmid
#67609)
-
Purposebacterial expression of GST-PRMT9 D258G Double E loop mutant
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 67609 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGEX-6p-1
-
Backbone manufacturerGE healthcare
- Backbone size w/o insert (bp) 4984
- Total vector size (bp) 7518
-
Modifications to backbonenone
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namePRMT9
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2538
-
MutationD258G double E loop mutant
-
GenBank IDNM_138364.3
-
Entrez GenePRMT9 (a.k.a. PRMT10)
- Promoter tac
-
Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer 5GEX
- 3′ sequencing primer 3GEX (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
GST-PRMT9 D258G mutant was a gift from Steven Clarke (Addgene plasmid # 67609 ; http://n2t.net/addgene:67609 ; RRID:Addgene_67609) -
For your References section:
Unique Features of Human Protein Arginine Methyltransferase 9 (PRMT9) and its Substrate RNA Splicing Factor SF3B2. Hadjikyriacou A, Yang Y, Espejo A, Bedford MT, Clarke SG. J Biol Chem. 2015 May 15. pii: jbc.M115.659433. 10.1074/jbc.M115.659433 PubMed 25979344