A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants.
Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X, Dong Z, Liu YG
Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 66187 | pYLCRISPR/Cas9Pubi-H | expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection |
| 66188 | pYLCRISPR/Cas9Pubi-B | expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection |
| 66189 | pYLCRISPR/Cas9P35S-H | expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection |
| 66190 | pYLCRISPR/Cas9P35S-B | expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection |
| 66191 | pYLCRISPR/Cas9P35S-N | expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection |
| 66192 | pYLsgRNA-OsU3m/LacZ | cloning of sgRNAs for expression in plants |
| 66193 | pYLsgRNA-OsU3m | cloning of sgRNAs for expression in plants |
| 66194 | pYLsgRNA-OsU6a | cloning of sgRNAs for expression in plants |
| 66195 | pYLsgRNA-OsU6a/LacZ | cloning of sgRNAs for expression in plants |
| 66196 | pYLsgRNA-OsU6b | cloning of sgRNAs for expression in plants |
| 66197 | pYLsgRNA-OsU6c | cloning of sgRNAs for expression in plants |
| 66198 | pYLsgRNA-AtU3b | cloning of sgRNAs for expression in plants |
| 66199 | pYLsgRNA-AtU3b/LacZ | cloning of sgRNAs for expression in plants |
| 66200 | pYLsgRNA-AtU3d | cloning of sgRNAs for expression in plants |
| 66201 | pYLsgRNA-AtU3d/LacZ | cloning of sgRNAs for expression in plants |
| 66202 | pYLsgRNA-AtU6-1 | cloning of sgRNAs for expression in plants |
| 66203 | pYLsgRNA-AtU6-29 | cloning of sgRNAs for expression in plants |
| 106331 | pYLCRISPR/Cas9pUbi-N | Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection |