Pak3
(Plasmid
#99510)
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Purposeto express Pak3 in mammalian system
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99510 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV- myc
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3791
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePak3
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SpeciesM. musculus (mouse)
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Insert Size (bp)1635
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Entrez GenePak3 (a.k.a. PAK-3, Pak65alpha, Pak65beta, Stk4)
- Promoter CMV
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pak3 was a gift from Dong-Yan Jin (Addgene plasmid # 99510 ; http://n2t.net/addgene:99510 ; RRID:Addgene_99510) -
For your References section:
Group I p21-activated kinases facilitate Tax-mediated transcriptional activation of the human T-cell leukemia virus type 1 long terminal repeats. Chan CP, Siu YT, Kok KH, Ching YP, Tang HM, Jin DY. Retrovirology. 2013 Apr 26;10:47. doi: 10.1186/1742-4690-10-47. 10.1186/1742-4690-10-47 PubMed 23622267
