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Addgene

pET21 E2F1
(Plasmid #90313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90313 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21c
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E2F1
  • Species
    H. sapiens (human)
  • GenBank ID
    NP_005216.1 NM_005225
  • Entrez Gene
    E2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)
  • Promoter T7
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer T7 terminator primer
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Joseph R. Nevins

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pcDNA3-HA-E2F1 was a generous gift from Dr. Nevins

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21 E2F1 was a gift from Maria Alvarado-Kristensson (Addgene plasmid # 90313 ; http://n2t.net/addgene:90313 ; RRID:Addgene_90313)

  • For your References section:

    Nuclear localization of gamma-tubulin affects E2F transcriptional activity and S-phase progression. Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M. FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 2011 Jul 25. 10.1096/fj.11-187484 PubMed 21788450
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