pPSU2
(Plasmid #89566)

• Purpose: High copy number plasmid 2 to prepare Penn State DNA molecular weight ladders
• Depositing Lab: Song Tan
• Publication: Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1.

Backbone

• Vector backbone: pUC9
• Backbone size w/o insert (bp): 2635
• Total vector size (bp): 7750
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: 750 bp EcoRV fragment
• Alt name: lambda DNA position 650-1399
• Species: phage lambda
• Insert Size (bp): 750
• GenBank ID: NC_001416.1

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: 3000 bp EcoRV fragment
• Alt name: lambda DNA position 25345-26210
• Species: phage lambda
• Insert Size (bp): 3000
• GenBank ID: NC_001416.1

Gene/Insert 3

• Gene/Insert name: 4000 bp EcoRV fragment
• Alt name: lambda DNA position 22948-24306
• Species: phage lambda
• Insert Size (bp): 4000
• GenBank ID: NC_001416.1

Gene/Insert 4

• Gene/Insert name: 50 bp PstI fragment
• Alt name: lambda DNA position 26172-26210
• Species: phage lambda
• Insert Size (bp): 50
• GenBank ID: NC_001416.1

Gene/Insert 5

• Gene/Insert name: 100 bp PstI fragment
• Alt name: lambda DNA position 2554-2656
• Species: phage lambda
• Insert Size (bp): 100
• GenBank ID: NC_001416.1

Cloning Information for Gene/Insert 5

• Cloning method: Restriction Enzyme
• 5′ cloning site: NgoMIV (not destroyed)
• 3′ cloning site: None (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 6

• Gene/Insert name: 200 bp PstI fragment
• Alt name: lambda DNA 37262-37455
• Species: phage lambda
• Insert Size (bp): 200
• GenBank ID: NC_001416.1

Gene/Insert 7

• Gene/Insert name: 300 bp PstI fragment
• Alt name: lambda DNA postion 3856-4149
• Species: phage lambda
• Insert Size (bp): 300
• GenBank ID: NC_001416.1

Cloning Information for Gene/Insert 7

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (not destroyed)
• 3′ cloning site: BsrGi (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 8

• Gene/Insert name: 400 bp PstI fragment
• Alt name: lambda DNA position 19833-20232
• Species: phage lambda
• Insert Size (bp): 400
• GenBank ID: NC_001416.1

Cloning Information for Gene/Insert 8

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: None (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 9

• Gene/Insert name: 500 bp PstI fragment
• Alt name: lambda DNA position 11835-12334
• Species: phage lambda
• Insert Size (bp): 500
• GenBank ID: NC_001416.1

Cloning Information for Gene/Insert 9

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 10

• Gene/Insert name: 600 bp PstI fragment
• Alt name: lambda DNA position 2858-3447
• Species: phage lambda
• Insert Size (bp): 600
• GenBank ID: NC_001416.1

Cloning Information for Gene/Insert 10

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (not destroyed)
• 3′ cloning site: BsrGi (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 11

• Gene/Insert name: 1500 bp Psti fragment
• Alt name: lambda DNA position 25340-26170 and 743-1399
• Species: phage lambda
• Insert Size (bp): 1500
• GenBank ID: NC_001416.1

Gene/Insert 12

• Gene/Insert name: 4100 bp PstI fragment
• Alt name: lambda DNA position 22948-24306
• Species: phage lambda
• Insert Size (bp): 4100
• GenBank ID: NC_001416.1

Resource Information

• Supplemental Documents:
  • Preparation of Penn State DNA ladders
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry