p5M-PR-B-1-688
(Plasmid #89115)

• Purpose: mammalian expression of human progesterone receptor amino acid residues 1-688 in pCMV5 with 5 restriction sites deleted in polylinker
• Depositing Lab: Elizabeth Wilson
• Publication: Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-63. doi: 10.1074/jbc.M111.244715. Epub 2011 Jul 5.

Backbone

• Vector backbone: pCMV5
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human progesterone receptor-B N-term+DNA binding domain
• Species: H. sapiens (human)
• Mutation: 5 restriction sites deleted in polylinker, contains aa 1-688
• Entrez Gene: PGR (a.k.a. NR3C3, PR)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 5 restriction sites deleted from the pCMV5 polylinker are HindIII, PstI, SalI, BamHI, and XbaI.