pcDNAmyc-hPR-B-486-933
(Plasmid
#89118)
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Purposemammalian expression of human progesterone receptor amino acid residues 486-933 with N-terminal myc tag, has PR DNA and ligand binding domains
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89118 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3-myc
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman progesterone receptor ligand binding domain
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SpeciesH. sapiens (human)
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Mutationcontains aa 486-933
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Entrez GenePGR (a.k.a. NR3C3, PR)
- Promoter CMV
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNAmyc-hPR-B-486-933 was a gift from Elizabeth Wilson (Addgene plasmid # 89118 ; http://n2t.net/addgene:89118 ; RRID:Addgene_89118) -
For your References section:
Gain in transcriptional activity by primate-specific coevolution of melanoma antigen-A11 and its interaction site in androgen receptor. Liu Q, Su S, Blackwelder AJ, Minges JT, Wilson EM. J Biol Chem. 2011 Aug 26;286(34):29951-63. doi: 10.1074/jbc.M111.244715. Epub 2011 Jul 5. 10.1074/jbc.M111.244715 PubMed 21730049