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Addgene

pcDNAmyc-hPR-B-486-933
(Plasmid #89118)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89118 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3-myc
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human progesterone receptor ligand binding domain
  • Species
    H. sapiens (human)
  • Mutation
    contains aa 486-933
  • Entrez Gene
    PGR (a.k.a. NR3C3, PR)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNAmyc-hPR-B-486-933 was a gift from Elizabeth Wilson (Addgene plasmid # 89118 ; http://n2t.net/addgene:89118 ; RRID:Addgene_89118)
  • For your References section:

    Gain in transcriptional activity by primate-specific coevolution of melanoma antigen-A11 and its interaction site in androgen receptor. Liu Q, Su S, Blackwelder AJ, Minges JT, Wilson EM. J Biol Chem. 2011 Aug 26;286(34):29951-63. doi: 10.1074/jbc.M111.244715. Epub 2011 Jul 5. 10.1074/jbc.M111.244715 PubMed 21730049