p5M-hPR-B
(Plasmid
#89114)
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Purposemammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89114 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV5
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman progesterone receptor-B
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SpeciesH. sapiens (human)
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Mutation5 restriction sites deleted in polylinker
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Entrez GenePGR (a.k.a. NR3C3, PR)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The 5 restriction sites deleted from the pCMV5 polylinker are HindIII, PstI, SalI, BamHI, and XbaI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5M-hPR-B was a gift from Elizabeth Wilson (Addgene plasmid # 89114 ; http://n2t.net/addgene:89114 ; RRID:Addgene_89114) -
For your References section:
Primate-specific melanoma antigen-A11 regulates isoform-specific human progesterone receptor-B transactivation. Su S, Blackwelder AJ, Grossman G, Minges JT, Yuan L, Young SL, Wilson EM. J Biol Chem. 2012 Oct 5;287(41):34809-24. doi: 10.1074/jbc.M112.372797. Epub 2012 Aug 13. 10.1074/jbc.M112.372797 PubMed 22891251