pcDNA flag PPAR gamma E499Q
(Plasmid #8896)

• Depositing Lab: Bruce Spiegelman
• Publication: Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-33.

Backbone

• Vector backbone: pcDNA3.1 myc-His A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPAR gamma
• Alt name: PPARg
• Species: M. musculus (mouse)
• Insert Size (bp): 1547
• Mutation: E499Q, a mutant in the AF2 domain. Binds PPARg ligands with comparable affininty as wild-type, but no ligand-dependent transcriptional activation.
• GenBank ID: NM_011146
• Entrez Gene: Pparg (a.k.a. Nr1c3, PPAR-gamma, PPAR-gamma2, PPARgamma, PPARgamma2)
• Tag / Fusion Protein:
  • flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry