TUBG1
(Plasmid #87954)

• Purpose: expresses human gamma-tubulin with a GFP-tag on the N-terminal of the protein
• Depositing Lab: Maria Alvarado-Kristensson
• Publication: Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eCollection 2016 Sep.

Backbone

• Vector backbone: pEGFP-C1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gamma-tubulin 1
• Alt name: TUBG1
• Species: H. sapiens (human)
• Insert Size (bp): 999
• Mutation: whole protein, The C-terminal is a sh-gamma-tubulin resistant gene. C 1236 and 1242 are mutated to T
• GenBank ID: NM_001070.4
• Entrez Gene: TUBG1 (a.k.a. CDCBM4, GCP-1, TUBG, TUBGCP1)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The TUBG1 was originally cloned by Dr. Jiri Bartek, I subclonned the gamma-tubulin gene into the pEGFN1 vector, and then mutated C 1236 and 1242 are mutated to T.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry