pCAG-VSFP2.3 rev. with Flex
(Plasmid #87948)

• Purpose: Plasmid used (after linearization) to generate transgenic mice that express the genetically encoded voltage indicator upon Cre recombination.
• Depositing Lab: Thomas Knopfel
• Publication: Quinn et al Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14852-14857. doi: 10.1073/pnas.1611184114. Epub 2016 Dec 7.

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 4779
• Total vector size (bp): 7979
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Voltage-Sensitive Fluorescent Protein 2.3
• Alt name: VSFP2.3
• Species: Synthetic
• Insert Size (bp): 2175
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoNI (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• Supplemental Documents:
  • RY.pCAG VSFP2.3 rev with Flex.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry