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Addgene

pLinker-AID-3xHA-HXGPRT-LoxP
(Plasmid #86553)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86553 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2830
  • Total vector size (bp) 2830
  • Vector type
    Expression in Toxoplasma gondii
  • Selectable markers
    HXGPR for Toxoplasma gondii

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Linker-AID-3xHA-HXGPRT 3'UTR
  • Species
    Toxoplasma gondii
  • Insert Size (bp)
    1287

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer attcgggcccgctagcAAGGGCTCGGGCTCGACCCAGCTGATGGGCAGTGTCGAGCT
  • 3′ sequencing primer ATACCGTCGACCCTCGAGTAGAACTAGTGGATCCGAGCACGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLinker-AID-3xHA-HXGPRT-LoxP was a gift from David Sibley (Addgene plasmid # 86553 ; http://n2t.net/addgene:86553 ; RRID:Addgene_86553)

  • For your References section:

    Calmodulin-like proteins localized to the conoid regulate motility and cell invasion by Toxoplasma gondii. Long S, Brown KM, Drewry LL, Anthony B, Phan IQH, Sibley LD. PLoS Pathog. 2017 May 5;13(5):e1006379. doi: 10.1371/journal.ppat.1006379. PPATHOGENS-D-17-00427 [pii] PubMed 28475612
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