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pMTS_mScarlet-i_N1
(Plasmid #85059)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85059 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmScarlet-i_N1
  • Backbone manufacturer
    Gadella Lab
  • Backbone size w/o insert (bp) 4739
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MTS
  • Alt name
    Mitochondrial Targeting Signal
  • Alt name
    COX8A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    87
  • Mutation
    aa1-29
  • Entrez Gene
    COX8A (a.k.a. COX, COX8, COX8-2, COX8L, MC4DN15, VIII, VIII-L)
  • Promoter CMV
  • Tag / Fusion Protein
    • mScarlet-i (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer AGGTCTATATAAGCAGAGC
  • 3′ sequencing primer TCTACAAATGTGGTATGGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid has extra sequence (T7 promoter) between CMV and the insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMTS_mScarlet-i_N1 was a gift from Dorus Gadella (Addgene plasmid # 85059 ; http://n2t.net/addgene:85059 ; RRID:Addgene_85059)

  • For your References section:

    mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr. Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. 10.1038/nmeth.4074 PubMed 27869816
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