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Addgene

pMTH PKC beta II
(Plasmid #8410)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8410 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMTH
  • Backbone size w/o insert (bp) 11500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rat Protein Kinase C beta II
  • Alt name
    PKC beta
  • Alt name
    PKCbII
  • Alt name
    Protein Kinase C beta
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Prkcb (a.k.a. Pkcb, Prkcb1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer MT1-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PKC-bII cDNA was a gift from Dr. John Knopf. The pMTH backbone was a gift from Dr. Michael Kuehl (backbone map uploaded here).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMTH PKC beta II was a gift from Frederic Mushinski (Addgene plasmid # 8410 ; http://n2t.net/addgene:8410 ; RRID:Addgene_8410)

  • For your References section:

    Phorbol ester-induced myeloid differentiation is mediated by protein kinase C-alpha and -delta and not by protein kinase C-beta II, -epsilon, -zeta, and -eta. Mischak H, Pierce JH, Goodnight J, Kazanietz MG, Blumberg PM, Mushinski JF. J Biol Chem 1993 Sep 25;268(27):20110-5. PubMed 8376369
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