pMTH PKC alpha
(Plasmid
#8409)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8409 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMTH
- Backbone size w/o insert (bp) 11500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMouse Protein Kinase C alpha
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Alt nameProtein Kinase C alpha
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Alt namePKC alpha
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SpeciesM. musculus (mouse)
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Insert Size (bp)2500
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GenBank IDM25811
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Entrez GenePrkca (a.k.a. Pkca)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe pMTH backbone was a gift from Dr. Michael Kuehl (backbone map uploaded here). PKC alpha was a gift from Dr. Stefan Rose-John.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMTH PKC alpha was a gift from Frederic Mushinski (Addgene plasmid # 8409 ; http://n2t.net/addgene:8409 ; RRID:Addgene_8409) -
For your References section:
Phorbol ester-induced myeloid differentiation is mediated by protein kinase C-alpha and -delta and not by protein kinase C-beta II, -epsilon, -zeta, and -eta. Mischak H, Pierce JH, Goodnight J, Kazanietz MG, Blumberg PM, Mushinski JF. J Biol Chem 1993 Sep 25;268(27):20110-5. PubMed 8376369
Map uploaded by the depositor.