pscAAV-CAG-GFP
(Plasmid #83279)

• Purpose: recombinant AAV vector packaging self-complementary GFP under the CAG promoter
• Depositing Lab: Mark Kay
• Publication: Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131610. doi: 10.1172/jci.insight.131610.

Backbone

• Vector backbone: pAAV with one trs deleted ITR and CAG promoter
• Backbone size w/o insert (bp): 2977
• Total vector size (bp): 4821
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Need to check for integrity of ITRs by restriction digest with AhdI, also with XmaI.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: enhanced green fluorescent protein
• Alt name: eGFP
• Species: Aequorea victoria
• Insert Size (bp): 720
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EagI (not destroyed)
• 5′ sequencing primer: GTTATTGTGCTGTCTCATC
• 3′ sequencing primer: CCACACCTCCCCCTGAAC

Resource Information

• Supplemental Documents:
  • pscAAV-CAG-GFP.dna
• A portion of this plasmid was derived from a plasmid made by: AAV backbone as well as eGFP sequences were obtained from Addgene plasmid #32396, the CAG promoter was obtained from Addgene plasmid #37825
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry