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pRetro-XT-PAK2-CFP(puro)-L107F
(Plasmid #81180)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRetroX-Tight-Pur
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6567
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p21 (RAC1) activated kinase 2
  • Alt name
    PAK2
  • Species
    H. sapiens (human)
  • Mutation
    L107F
  • Entrez Gene
    PAK2 (a.k.a. KNO2, PAK65, PAKgamma)
  • Promoter P-tight TRE
  • Tag / Fusion Protein
    • CFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site NruI (unknown if destroyed)
  • 5′ sequencing primer pBABE 5
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a coding difference of L106F rather than L107F in the PAK2 insert compared to NP_002568.2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetro-XT-PAK2-CFP(puro)-L107F was a gift from Angeliki Malliri (Addgene plasmid # 81180 ; http://n2t.net/addgene:81180 ; RRID:Addgene_81180)

  • For your References section:

    Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Whalley HJ, Porter AP, Diamantopoulou Z, White GR, Castaneda-Saucedo E, Malliri A. Nat Commun. 2015 Jun 16;6:7437. doi: 10.1038/ncomms8437. 10.1038/ncomms8437 PubMed 26078008
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