pXPG
(Plasmid #71248)

• Purpose: (Empty Backbone) improved luciferase reporter gene plasmid
• Depositing Lab: Peter Cockerill
• Publication: Bert et al Plasmid. 2000 Sep;44(2):173-82.

Backbone

• Vector backbone: pXP1 and pGL3
• Backbone manufacturer: Nordeen, 1988 and Promega
• Modifications to backbone: A single C to A mutation 564 bp upstream of the origin of replication at plasmid position 4660 was made to create pXPM. A HindIII/XbaI fragment of pGL3 (Promega) containing the improved luciferase gene Luc1 (Sherf et al.,1994) was cloned into into HindIII/XbaI-cut pXPM
• Vector type: Mammalian Expression, Luciferase
• Promoter: none
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 3′ sequencing primer: LucNRev

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor states "A spontaneous single C to A mutation was identified 564 bp upstream of the origin of replication at plasmid position 4660 in pXPM which generated high copy replication. A HindIII/XbaI fragment of pGL3 (Promega) containing the improved luciferase gene Luc+ (Sherf et al.,1994) was cloned into into HindIII/XbaI-cut pXPM."

JM109 are the cells used by the author for pXPG and its derivatives.