pRL1342
(Plasmid #70691)

• Purpose: (Empty Backbone) RSF1010-based vector with extensive polylinker, and resistance to Cm and Em. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.
• Depositing Lab: Peter Wolk
• Publication: Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63. Epub 2007 Jul 17.

Backbone

• Vector backbone: RSF1010
• Backbone size (bp): 8416
• Vector type: Bacterial shuttle vector
• Selectable markers: Erythromycin for cyanobacteria

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: RSF1010 from Michael Bagdasarian
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MCS: (PT7→)HindIII-SphI-PstI(Sse8381)-SalI-KpnI-SacI-ClaI-ApaI-SacII-NsiI-NdeI-BglII-AflII-StuI-AvrII-XbaI-AatII-BsiWI-ApaLI-XhoI(PspXI)-SrfI-AscI-RsrII-NaeI-BamHI-(←PSP6)