SP-dCas9-VPR Citations (106)
Originally described in: Highly efficient Cas9-mediated transcriptional programming.Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. PubMed Journal
Articles Citing SP-dCas9-VPR
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Cas9 gRNA engineering for genome editing, activation and repression.
Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR, Collins JJ, Weiss R, Church G.
Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
PubMed
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| Potent and Targeted Activation of Latent HIV-1 Using the CRISPR/dCas9 Activator Complex. Saayman SM, Lazar DC, Scott TA, Hart JR, Takahashi M, Burnett JC, Planelles V, Morris KV, Weinberg MS. Mol Ther. 2015 Nov 19. doi: 10.1038/mt.2015.202. PubMed |
| Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Jusiak B, Cleto S, Perez-Pinera P, Lu TK. Trends Biotechnol. 2016 Jan 22. pii: S0167-7799(15)00274-7. doi: 10.1016/j.tibtech.2015.12.014. PubMed |
Comparison of Cas9 activators in multiple species.
Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church G.
Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
PubMed
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| Precision Modulation of Neurodegenerative Disease-Related Gene Expression in Human iPSC-Derived Neurons. Heman-Ackah SM, Bassett AR, Wood MJ. Sci Rep. 2016 Jun 24;6:28420. doi: 10.1038/srep28420. PubMed |
| Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system. Garcia-Bloj B, Moses C, Sgro A, Plani-Lam J, Arooj M, Duffy C, Thiruvengadam S, Sorolla A, Rashwan R, Mancera RL, Leisewitz A, Swift-Scanlan T, Corvalan AH, Blancafort P. Oncotarget. 2016 Sep 13;7(37):60535-60554. doi: 10.18632/oncotarget.11142. PubMed |
| Targeted Gene Activation Using RNA-Guided Nucleases. Brown A, Woods WS, Perez-Pinera P. Methods Mol Biol. 2017;1468:235-50. doi: 10.1007/978-1-4939-4035-6_16. PubMed |
| Metabolic gatekeeper function of B-lymphoid transcription factors. Chan LN, Chen Z, Braas D, Lee JW, Xiao G, Geng H, Cosgun KN, Hurtz C, Shojaee S, Cazzaniga V, Schjerven H, Ernst T, Hochhaus A, Kornblau SM, Konopleva M, Pufall MA, Cazzaniga G, Liu GJ, Milne TA, Koeffler HP, Ross TS, Sanchez-Garcia I, Borkhardt A, Yamamoto KR, Dickins RA, Graeber TG, Muschen M. Nature. 2017 Feb 23;542(7642):479-483. doi: 10.1038/nature21076. Epub 2017 Feb 13. PubMed |
Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes.
Ho SM, Hartley BJ, Flaherty E, Rajarajan P, Abdelaal R, Obiorah I, Barretto N, Muhammad H, Phatnani HP, Akbarian S, Brennand KJ.
Stem Cell Reports. 2017 Aug 8;9(2):615-628. doi: 10.1016/j.stemcr.2017.06.012. Epub 2017 Jul 27.
PubMed
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| Effect of ELOVL3 expression on bovine skeletal muscle-derived satellite cell differentiation. Liu D, Xu JH, Tong HL, Li SF, Yan YQ. Biochem Biophys Res Commun. 2017 Dec 2;493(4):1457-1463. doi: 10.1016/j.bbrc.2017.07.118. Epub 2017 Aug 3. PubMed |
Multiplexed sgRNA Expression Allows Versatile Single Nonrepetitive DNA Labeling and Endogenous Gene Regulation.
Shao S, Chang L, Sun Y, Hou Y, Fan X, Sun Y.
ACS Synth Biol. 2017 Sep 7. doi: 10.1021/acssynbio.7b00268.
PubMed
Associated Plasmids |
| CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. Nat Methods. 2017 Oct;14(10):963-966. doi: 10.1038/nmeth.4430. Epub 2017 Sep 11. PubMed |
| dCas9-mediated transcriptional activation of tissue inhibitor of metalloproteinases. Duellman T, Doll A, Chen X, Wakamiya R, Yang J. Metalloproteinases Med. 2017;4:63-73. doi: 10.2147/MNM.S146752. Epub 2017 Sep 19. PubMed |
Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors.
Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman JS, Joung JK.
Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483.
PubMed
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| Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors. Kleinjan DA, Wardrope C, Nga Sou S, Rosser SJ. Nat Commun. 2017 Oct 30;8(1):1191. doi: 10.1038/s41467-017-01222-y. PubMed |
In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.
Liao HK, Hatanaka F, Araoka T, Reddy P, Wu MZ, Sui Y, Yamauchi T, Sakurai M, O'Keefe DD, Nunez-Delicado E, Guillen P, Campistol JM, Wu CJ, Lu LF, Esteban CR, Izpisua Belmonte JC.
Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7.
PubMed
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| ciRS-7 exonic sequence is embedded in a long non-coding RNA locus. Barrett SP, Parker KR, Horn C, Mata M, Salzman J. PLoS Genet. 2017 Dec 13;13(12):e1007114. doi: 10.1371/journal.pgen.1007114. eCollection 2017 Dec. PubMed |
Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing.
Lu J, Zhao C, Zhao Y, Zhang J, Zhang Y, Chen L, Han Q, Ying Y, Peng S, Ai R, Wang Y.
Nucleic Acids Res. 2018 Mar 16;46(5):e25. doi: 10.1093/nar/gkx1222.
PubMed
Associated Plasmids |
| Mechanogenetics for the remote and noninvasive control of cancer immunotherapy. Pan Y, Yoon S, Sun J, Huang Z, Lee C, Allen M, Wu Y, Chang YJ, Sadelain M, Shung KK, Chien S, Wang Y. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):992-997. doi: 10.1073/pnas.1714900115. Epub 2018 Jan 17. PubMed |
| Next-generation CRISPR/Cas9 transcriptional activation in Drosophila using flySAM. Jia Y, Xu RG, Ren X, Ewen-Campen B, Rajakumar R, Zirin J, Yang-Zhou D, Zhu R, Wang F, Mao D, Peng P, Qiao HH, Wang X, Liu LP, Xu B, Ji JY, Liu Q, Sun J, Perrimon N, Ni JQ. Proc Natl Acad Sci U S A. 2018 May 1;115(18):4719-4724. doi: 10.1073/pnas.1800677115. Epub 2018 Apr 16. PubMed |
| CRISPR-Mediated Reactivation of DKK3 Expression Attenuates TGF-beta Signaling in Prostate Cancer. Kardooni H, Gonzalez-Gualda E, Stylianakis E, Saffaran S, Waxman J, Kypta RM. Cancers (Basel). 2018 May 28;10(6). pii: cancers10060165. doi: 10.3390/cancers10060165. PubMed |
| Targeted Reactivation of FMR1 Transcription in Fragile X Syndrome Embryonic Stem Cells. Haenfler JM, Skariah G, Rodriguez CM, Monteiro da Rocha A, Parent JM, Smith GD, Todd PK. Front Mol Neurosci. 2018 Aug 15;11:282. doi: 10.3389/fnmol.2018.00282. eCollection 2018. PubMed |
| Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression. Srikanth P, Lagomarsino VN, Pearse RV 2nd, Liao M, Ghosh S, Nehme R, Seyfried N, Eggan K, Young-Pearse TL. Transl Psychiatry. 2018 Nov 8;8(1):245. doi: 10.1038/s41398-018-0281-9. PubMed |
| Long Non-Coding RNA Modulation of VEGF-A during Hypoxia. Nieminen T, Scott TA, Lin FM, Chen Z, Yla-Herttuala S, Morris KV. Noncoding RNA. 2018 Nov 20;4(4). pii: ncrna4040034. doi: 10.3390/ncrna4040034. PubMed |
| A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9. Fang L, Hung SSC, Yek J, El Wazan L, Nguyen T, Khan S, Lim SY, Hewitt AW, Wong RCB. Mol Ther Nucleic Acids. 2019 Mar 1;14:184-191. doi: 10.1016/j.omtn.2018.11.008. Epub 2018 Nov 20. PubMed |
Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins.
Mangeot PE, Risson V, Fusil F, Marnef A, Laurent E, Blin J, Mournetas V, Massourides E, Sohier TJM, Corbin A, Aube F, Teixeira M, Pinset C, Schaeffer L, Legube G, Cosset FL, Verhoeyen E, Ohlmann T, Ricci EP.
Nat Commun. 2019 Jan 3;10(1):45. doi: 10.1038/s41467-018-07845-z.
PubMed
Associated Plasmids |
| Podocan affects C2C12 myogenic differentiation by enhancing Wnt/beta-catenin signaling. Liu D, Li S, Cui Y, Tong H, Li S, Yan Y. J Cell Physiol. 2019 Jul;234(7):11130-11139. doi: 10.1002/jcp.27763. Epub 2019 Jan 16. PubMed |
| Activating PTEN Tumor Suppressor Expression with the CRISPR/dCas9 System. Moses C, Nugent F, Waryah CB, Garcia-Bloj B, Harvey AR, Blancafort P. Mol Ther Nucleic Acids. 2019 Mar 1;14:287-300. doi: 10.1016/j.omtn.2018.12.003. Epub 2018 Dec 14. PubMed |
| Multiplexed and tunable transcriptional activation by promoter insertion using nuclease-assisted vector integration. Brown A, Winter J, Gapinske M, Tague N, Woods WS, Perez-Pinera P. Nucleic Acids Res. 2019 Jul 9;47(12):e67. doi: 10.1093/nar/gkz210. PubMed |
| Tailoring the CRISPR system to transactivate coagulation gene promoters in normal and mutated contexts. Pignani S, Zappaterra F, Barbon E, Follenzi A, Bovolenta M, Bernardi F, Branchini A, Pinotti M. Biochim Biophys Acta Gene Regul Mech. 2019 Jun;1862(6):619-624. doi: 10.1016/j.bbagrm.2019.04.002. Epub 2019 Apr 18. PubMed |
| Three-Component Repurposed Technology for Enhanced Expression: Highly Accumulable Transcriptional Activators via Branched Tag Arrays. Kunii A, Hara Y, Takenaga M, Hattori N, Fukazawa T, Ushijima T, Yamamoto T, Sakuma T. CRISPR J. 2018 Oct;1:337-347. doi: 10.1089/crispr.2018.0009. PubMed |
| GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway. Li S, Fu Y, Pang Y, Tong H, Li S, Yan Y. J Cell Physiol. 2019 Nov;234(11):21211-21223. doi: 10.1002/jcp.28727. Epub 2019 Apr 25. PubMed |
| Identification of on-target mutagenesis during correction of a beta-thalassemia splice mutation in iPS cells with optimised CRISPR/Cas9-double nickase reveals potential safety concerns. Alateeq S, Ovchinnikov D, Tracey T, Whitworth D, Al-Rubaish A, Al-Ali A, Wolvetang E. APL Bioeng. 2018 Dec 3;2(4):046103. doi: 10.1063/1.5048625. eCollection 2018 Dec. PubMed |
| Three-dimensional analysis of single molecule FISH in human colon organoids. Omerzu M, Fenderico N, de Barbanson B, Sprangers J, de Ridder J, Maurice MM. Biol Open. 2019 Aug 9;8(8). pii: bio.042812. doi: 10.1242/bio.042812. PubMed |
| Targeted Activation of Cystic Fibrosis Transmembrane Conductance Regulator. Villamizar O, Waters SA, Scott T, Saayman S, Grepo N, Urak R, Davis A, Jaffe A, Morris KV. Mol Ther. 2019 Oct 2;27(10):1737-1748. doi: 10.1016/j.ymthe.2019.07.002. Epub 2019 Jul 15. PubMed |
| Coordinate regulation of ELF5 and EHF at the chr11p13 CF modifier region. Swahn H, Sabith Ebron J, Lamar KM, Yin S, Kerschner JL, NandyMazumdar M, Coppola C, Mendenhall EM, Leir SH, Harris A. J Cell Mol Med. 2019 Nov;23(11):7726-7740. doi: 10.1111/jcmm.14646. Epub 2019 Sep 26. PubMed |
| Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6. Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A. Mol Cell. 2019 Dec 19;76(6):922-937.e7. doi: 10.1016/j.molcel.2019.09.012. Epub 2019 Oct 8. PubMed |
| Induced cardiomyocyte maturation: Cardiac transcription factors are necessary but not sufficient. Dal-Pra S, Hodgkinson CP, Dzau VJ. PLoS One. 2019 Oct 17;14(10):e0223842. doi: 10.1371/journal.pone.0223842. eCollection 2019. PubMed |
| Off-target effects of CRISPRa on interleukin-6 expression. Soubeyrand S, Lau P, Peters V, McPherson R. PLoS One. 2019 Oct 28;14(10):e0224113. doi: 10.1371/journal.pone.0224113. eCollection 2019. PubMed |
| Hypoxia drives glucose transporter 3 expression through HIF-mediated induction of the long non-coding RNA NICI. Lauer V, Grampp S, Platt J, Lafleur V, Lombardi O, Choudhry H, Kranz F, Hartmann A, Wullich B, Yamamoto A, Coleman ML, Ratcliffe PJ, Mole DR, Schodel J. J Biol Chem. 2019 Nov 5. pii: RA119.009827. doi: 10.1074/jbc.RA119.009827. PubMed |
| CRISPR-Mediated Activation of Endogenous Gene Expression in the Postnatal Heart. Schoger E, Carroll KJ, Iyer LM, McAnally JR, Tan W, Liu N, Noack C, Shomroni O, Salinas G, Gross J, Herzog N, Doroudgar S, Bassel-Duby R, Zimmermann WH, Zelarayan LC. Circ Res. 2020 Jan 3;126(1):6-24. doi: 10.1161/CIRCRESAHA.118.314522. Epub 2019 Nov 15. PubMed |
| Targeted regulation of fibroblast state by CRISPR-mediated CEBPA expression. Liu W, Meridew JA, Aravamudhan A, Ligresti G, Tschumperlin DJ, Tan Q. Respir Res. 2019 Dec 11;20(1):281. doi: 10.1186/s12931-019-1253-1. PubMed |
| A functional variant near XCL1 gene improves breast cancer survival via promoting cancer immunity. Chou WC, Hsiung CN, Chen WT, Tseng LM, Wang HC, Chu HW, Hou MF, Yu JC, Shen CY. Int J Cancer. 2020 Apr 15;146(8):2182-2193. doi: 10.1002/ijc.32855. Epub 2020 Jan 25. PubMed |
A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells.
Hazelbaker DZ, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D, Eggan K, Barrett LE.
Sci Rep. 2020 Jan 20;10(1):635. doi: 10.1038/s41598-020-57500-1.
PubMed
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| TCP11L2 promotes bovine skeletal muscle-derived satellite cell migration and differentiation via FMNL2. Li S, Wang Z, Tong H, Li S, Yan Y. J Cell Physiol. 2020 Oct;235(10):7183-7193. doi: 10.1002/jcp.29617. Epub 2020 Feb 4. PubMed |
Multiplexed Gene Expression Tuning with Orthogonal Synthetic Gene Circuits.
Szenk M, Yim T, Balazsi G.
ACS Synth Biol. 2020 Apr 17;9(4):930-939. doi: 10.1021/acssynbio.9b00534. Epub 2020 Mar 23.
PubMed
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| A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. Xi H, Langerman J, Sabri S, Chien P, Young CS, Younesi S, Hicks M, Gonzalez K, Fujiwara W, Marzi J, Liebscher S, Spencer M, Van Handel B, Evseenko D, Schenke-Layland K, Plath K, Pyle AD. Cell Stem Cell. 2020 Jul 2;27(1):158-176.e10. doi: 10.1016/j.stem.2020.04.017. Epub 2020 May 11. PubMed |
| Sense-antisense miRNA pairs constitute an elaborate reciprocal regulatory circuit. Song Y, Li L, Yang W, Fu Q, Chen W, Fang Z, Li W, Gu N, Zhang R. Genome Res. 2020 May;30(5):661-672. doi: 10.1101/gr.257121.119. Epub 2020 May 18. PubMed |
| CRISPR/dCas9-based Scn1a gene activation in inhibitory neurons ameliorates epileptic and behavioral phenotypes of Dravet syndrome model mice. Yamagata T, Raveau M, Kobayashi K, Miyamoto H, Tatsukawa T, Ogiwara I, Itohara S, Hensch TK, Yamakawa K. Neurobiol Dis. 2020 Jul;141:104954. doi: 10.1016/j.nbd.2020.104954. Epub 2020 May 21. PubMed |
| Deciphering the Ets-1/2-mediated transcriptional regulation of F8 gene identifies a minimal F8 promoter for hemophilia A gene therapy. Fama R, Borroni E, Merlin S, Airoldi C, Pignani S, Cucci A, Cora D, Bruscaggin V, Scardellato S, Faletti S, Pelicci G, Pinotti M, Walker GE, Follenzi A. Haematologica. 2020 May 28. pii: haematol.2019.239202. doi: 10.3324/haematol.2019.239202. PubMed |
| Localized delivery of CRISPR/dCas9 via layer-by-layer self-assembling peptide coating on nanofibers for neural tissue engineering. Zhang K, Chooi WH, Liu S, Chin JS, Murray A, Nizetic D, Cheng D, Chew SY. Biomaterials. 2020 Oct;256:120225. doi: 10.1016/j.biomaterials.2020.120225. Epub 2020 Jul 8. PubMed |
A gene therapy for inherited blindness using dCas9-VPR-mediated transcriptional activation.
Bohm S, Splith V, Riedmayr LM, Rotzer RD, Gasparoni G, Nordstrom KJV, Wagner JE, Hinrichsmeyer KS, Walter J, Wahl-Schott C, Fenske S, Biel M, Michalakis S, Becirovic E.
Sci Adv. 2020 Aug 19;6(34):eaba5614. doi: 10.1126/sciadv.aba5614. eCollection 2020 Aug.
PubMed
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| Cis-acting lnc-eRNA SEELA directly binds histone H4 to promote histone recognition and leukemia progression. Fang K, Huang W, Sun YM, Chen TQ, Zeng ZC, Yang QQ, Pan Q, Han C, Sun LY, Luo XQ, Wang WT, Chen YQ. Genome Biol. 2020 Nov 3;21(1):269. doi: 10.1186/s13059-020-02186-x. PubMed |
| Identification of Long Noncoding RNAs Involved in Differentiation and Survival of Vascular Smooth Muscle Cells. Lim YH, Ryu J, Kook H, Kim YK. Mol Ther Nucleic Acids. 2020 Aug 29;22:209-221. doi: 10.1016/j.omtn.2020.08.032. eCollection 2020 Dec 4. PubMed |
| Broadly active zinc finger protein-guided transcriptional activation of HIV-1. Scott TA, O'Meally D, Grepo NA, Soemardy C, Lazar DC, Zheng Y, Weinberg MS, Planelles V, Morris KV. Mol Ther Methods Clin Dev. 2020 Oct 27;20:18-29. doi: 10.1016/j.omtm.2020.10.018. eCollection 2021 Mar 12. PubMed |
Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells.
Xi H, Young CS, Pyle AD.
STAR Protoc. 2020 Nov 5;1(3):100158. doi: 10.1016/j.xpro.2020.100158. eCollection 2020 Dec 18.
PubMed
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| The BRCA1 Pseudogene Negatively Regulates Antitumor Responses through Inhibition of Innate Immune Defense Mechanisms. Han YJ, Zhang J, Lee JH, Mason JM, Karginova O, Yoshimatsu TF, Hao Q, Hurley I, Brunet LP, Prat A, Prasanth KV, Gack MU, Olopade OI. Cancer Res. 2021 Mar 15;81(6):1540-1551. doi: 10.1158/0008-5472.CAN-20-1959. Epub 2021 Jan 20. PubMed |
Model-guided design of mammalian genetic programs.
Muldoon JJ, Kandula V, Hong M, Donahue PS, Boucher JD, Bagheri N, Leonard JN.
Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe9375. doi: 10.1126/sciadv.abe9375. Print 2021 Feb.
PubMed
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| Integrative analysis of liver-specific non-coding regulatory SNPs associated with the risk of coronary artery disease. Selvarajan I, Toropainen A, Garske KM, Lopez Rodriguez M, Ko A, Miao Z, Kaminska D, Ounap K, Ord T, Ravindran A, Liu OH, Moreau PR, Jawahar Deen A, Mannisto V, Pan C, Levonen AL, Lusis AJ, Heikkinen S, Romanoski CE, Pihlajamaki J, Pajukanta P, Kaikkonen MU. Am J Hum Genet. 2021 Mar 4;108(3):411-430. doi: 10.1016/j.ajhg.2021.02.006. Epub 2021 Feb 23. PubMed |
| Structural variant evolution after telomere crisis. Dewhurst SM, Yao X, Rosiene J, Tian H, Behr J, Bosco N, Takai KK, de Lange T, Imielinski M. Nat Commun. 2021 Apr 7;12(1):2093. doi: 10.1038/s41467-021-21933-7. PubMed |
Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing.
Nuñez JK, Chen J, Pommier GC, Cogan JZ, Replogle JM, Adriaens C, Ramadoss GN, Shi Q, Hung KL, Samelson AJ, Pogson AN, Kim JYS, Chung A, Leonetti MD, Chang HY, Kampmann M, Bernstein BE, Hovestadt V, Gilbert LA, Weissman JS.
Cell. 2021 Apr 7. pii: S0092-8674(21)00353-6. doi: 10.1016/j.cell.2021.03.025.
PubMed
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| RNA transcripts stimulate homologous recombination by forming DR-loops. Ouyang J, Yadav T, Zhang JM, Yang H, Rheinbay E, Guo H, Haber DA, Lan L, Zou L. Nature. 2021 Jun;594(7862):283-288. doi: 10.1038/s41586-021-03538-8. Epub 2021 May 12. PubMed |
| Lupus susceptibility region containing CDKN1B rs34330 mechanistically influences expression and function of multiple target genes, also linked to proliferation and apoptosis. Singh B, Maiti GP, Zhou X, Fazel-Najafabadi M, Bae SC, Sun C, Terao C, Okada Y, Chua KH, Kochi Y, Guthridge JM, Zhang H, Weirauch M, James JA, Harley JB, Varshney GK, Looger LL, Nath SK. Arthritis Rheumatol. 2021 May 13. doi: 10.1002/art.41799. PubMed |
| Transcriptional Activation of Heterochromatin by Recruitment of dCas9 Activators. Frank L, Weinmann R, Erdel F, Trojanowski J, Rippe K. Methods Mol Biol. 2021;2351:307-320. doi: 10.1007/978-1-0716-1597-3_17. PubMed |
| Transposable elements and their KZFP controllers are drivers of transcriptional innovation in the developing human brain. Playfoot CJ, Duc J, Sheppard S, Dind S, Coudray A, Planet E, Trono D. Genome Res. 2021 Sep;31(9):1531-1545. doi: 10.1101/gr.275133.120. Epub 2021 Aug 16. PubMed |
Targeted regulation of transcription in primary cells using CRISPRa and CRISPRi.
Jensen TI, Mikkelsen NS, Gao Z, Fosselteder J, Pabst G, Axelgaard E, Laustsen A, Konig S, Reinisch A, Bak RO.
Genome Res. 2021 Nov;31(11):2120-2130. doi: 10.1101/gr.275607.121. Epub 2021 Aug 18.
PubMed
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Find and cut-and-transfer (FiCAT) mammalian genome engineering.
Pallares-Masmitja M, Ivancic D, Mir-Pedrol J, Jaraba-Wallace J, Tagliani T, Oliva B, Rahmeh A, Sanchez-Mejias A, Guell M.
Nat Commun. 2021 Dec 3;12(1):7071. doi: 10.1038/s41467-021-27183-x.
PubMed
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| Taurine stimulates protein synthesis and proliferation of C2C12 myoblast cells through the PI3K-ARID4B-mTOR pathway. Hao Q, Wang L, Zhang M, Wang Z, Li M, Gao X. Br J Nutr. 2021 Dec 9:1-12. doi: 10.1017/S0007114521004918. PubMed |
| Four-dimensional nuclear speckle phase separation dynamics regulate proteostasis. Dion W, Ballance H, Lee J, Pan Y, Irfan S, Edwards C, Sun M, Zhang J, Zhang X, Liu S, Zhu B. Sci Adv. 2022 Jan 7;8(1):eabl4150. doi: 10.1126/sciadv.abl4150. Epub 2022 Jan 5. PubMed |
| Deciphering the Design Rules of Toehold-Gated sgRNA for Conditional Activation of Gene Expression and Protein Degradation in Mammalian Cells. Hunt VM, Chen W. ACS Synth Biol. 2022 Jan 21;11(1):397-405. doi: 10.1021/acssynbio.1c00479. Epub 2022 Jan 7. PubMed |
| A Versatile Clustered Regularly Interspaced Palindromic Repeats Toolbox to Study Neurological CaV3.2 Channelopathies by Promoter-Mediated Transcription Control. Tsortouktzidis D, Troscher AR, Schulz H, Opitz T, Schoch S, Becker AJ, van Loo KMJ. Front Mol Neurosci. 2022 Jan 6;14:667143. doi: 10.3389/fnmol.2021.667143. eCollection 2021. PubMed |
| dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy. Riedmayr LM, Hinrichsmeyer KS, Karguth N, Bohm S, Splith V, Michalakis S, Becirovic E. Nat Protoc. 2022 Mar;17(3):781-818. doi: 10.1038/s41596-021-00666-3. Epub 2022 Feb 7. PubMed |
| Robust, Durable Gene Activation In Vivo via mRNA-Encoded Activators. Beyersdorf JP, Bawage S, Iglesias N, Peck HE, Hobbs RA, Wroe JA, Zurla C, Gersbach CA, Santangelo PJ. ACS Nano. 2022 Mar 31. doi: 10.1021/acsnano.1c10631. PubMed |
| Parkinson's disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons. Giehrl-Schwab J, Giesert F, Rauser B, Lao CL, Hembach S, Lefort S, Ibarra IL, Koupourtidou C, Luecken MD, Truong DJ, Fischer-Sternjak J, Masserdotti G, Prakash N, Ninkovic J, Holter SM, Vogt Weisenhorn DM, Theis FJ, Gotz M, Wurst W. EMBO Mol Med. 2022 May 9;14(5):e14797. doi: 10.15252/emmm.202114797. Epub 2022 Apr 4. PubMed |
| Systematic HIV-1 promoter targeting with CRISPR/dCas9-VPR reveals optimal region for activation of the latent provirus. Klinnert S, Chemnitzer A, Rusert P, Metzner KJ. J Gen Virol. 2022 Jun;103(6). doi: 10.1099/jgv.0.001754. PubMed |
| Control of CRK-RAC1 activity by the miR-1/206/133 miRNA family is essential for neuromuscular junction function. Klockner I, Schutt C, Gerhardt T, Boettger T, Braun T. Nat Commun. 2022 Jun 8;13(1):3180. doi: 10.1038/s41467-022-30778-7. PubMed |
| PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency. Lau CH, Huang S, Lam RHW, Tin C. Mol Ther Methods Clin Dev. 2022 May 29;26:26-37. doi: 10.1016/j.omtm.2022.05.005. eCollection 2022 Sep 8. PubMed |
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