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CA-RIT-NFAT1 (PGK-Puro)
(Plasmid #63214)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63214 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CA-RIT-NFAT1
  • Alt name
    constitutively active NFAT1 with RIT mutations
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2800
  • Mutation
    Amino acids 1-3 removed; CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP mutated to APRTAPIMAP, SPQRSRSPSPQPSP mutated to APQRARSPAPQPAP. RIT:R468A, I469A, T535G
  • Entrez Gene
    Nfatc2 (a.k.a. AI607462, NF-ATc2, NF-ATp, NFAT1, NFAT1-D, Nfatp)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer pBABE-5
  • 3′ sequencing primer MSCV-rev (5'-CAGCGGGGCTGCTAAAGCGCATGC-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

RIT mutations (R468, I469, and T535) selectively interfere with NFAT:AP-1 interaction

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CA-RIT-NFAT1 (PGK-Puro) was a gift from Anjana Rao (Addgene plasmid # 63214 ; http://n2t.net/addgene:63214 ; RRID:Addgene_63214)

  • For your References section:

    The Transcription Factor NFAT Promotes Exhaustion of Activated CD8 T Cells. Martinez GJ, Pereira RM, Aijo T, Kim EY, Marangoni F, Pipkin ME, Togher S, Heissmeyer V, Zhang YC, Crotty S, Lamperti ED, Ansel KM, Mempel TR, Lahdesmaki H, Hogan PG, Rao A. Immunity. 2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 10.1016/j.immuni.2015.01.006. 10.1016/j.immuni.2015.01.006 PubMed 25680272
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