CA-RIT-NFAT1 (PGK-Puro)
(Plasmid #63214)

• Purpose: Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interactio. Contains Puromycin selection marker
• Depositing Lab: Anjana Rao
• Publication: Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 10.1016/j.immuni.2015.01.006.

Backbone

• Vector backbone: unknown
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CA-RIT-NFAT1
• Alt name: constitutively active NFAT1 with RIT mutations
• Species: M. musculus (mouse)
• Insert Size (bp): 2800
• Mutation: Amino acids 1-3 removed; CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP mutated to APRTAPIMAP, SPQRSRSPSPQPSP mutated to APQRARSPAPQPAP. RIT:R468A, I469A, T535G
• Entrez Gene: Nfatc2 (a.k.a. AI607462, NF-ATc2, NF-ATp, NFAT1, NFAT1-D, Nfatp)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pBABE-5
• 3′ sequencing primer: MSCV-rev (5'-CAGCGGGGCTGCTAAAGCGCATGC-3')

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

RIT mutations (R468, I469, and T535) selectively interfere with NFAT:AP-1 interaction