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PurposeConstitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA binding
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRV
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Backbone manufacturerAnjana Rao lab
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 9000
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Vector typeMammalian Expression, Retroviral
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Selectable markersIRES-GFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFAT1
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Alt nameconstitutively active NFAT1 with RIT and DBD mutations
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SpeciesM. musculus (mouse)
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Insert Size (bp)2800
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MutationCA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP mutated to APRTAPIMAP, SPQRSRSPSPQPSP mutated to APQRARSPAPQPAP. RIT:R468A, I469A, T535G; DBD mutations: R423A, Y426A, E429A, G430A
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Entrez GeneNfatc2 (a.k.a. AI607462, NF-ATc2, NF-ATp, NFAT1, NFAT1-D, Nfatp)
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Tag
/ Fusion Protein
- 3x HA (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pBABE-5
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DBDmut-CA-RIT-NFAT1 was a gift from Anjana Rao (Addgene plasmid # 63669 ; http://n2t.net/addgene:63669 ; RRID:Addgene_63669) -
For your References section:
The Transcription Factor NFAT Promotes Exhaustion of Activated CD8 T Cells. Martinez GJ, Pereira RM, Aijo T, Kim EY, Marangoni F, Pipkin ME, Togher S, Heissmeyer V, Zhang YC, Crotty S, Lamperti ED, Ansel KM, Mempel TR, Lahdesmaki H, Hogan PG, Rao A. Immunity. 2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 10.1016/j.immuni.2015.01.006. 10.1016/j.immuni.2015.01.006 PubMed 25680272