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Addgene

AI-CN590
(Plasmid #62596)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62596 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 3671
  • Total vector size (bp) 6277
  • Vector type
    Mammalian Expression, TALEN ; homology-directed repair donor
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cerulean-P2A-PuroR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2606
  • GenBank ID
  • Entrez Gene
    KCTD13 (a.k.a. BACURD1, FKSG86, PDIP1, POLDIP1, hBACURD1)
  • Promoter hEF1alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer EGFP-N
  • 3′ sequencing primer Bglob-pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains the Cerulean fluorescent protein and loxP sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AI-CN590 was a gift from Joshua Grimley (Addgene plasmid # 62596 ; http://n2t.net/addgene:62596 ; RRID:Addgene_62596)

  • For your References section:

    Genome engineering of isogenic human ES cells to model autism disorders. Martinez RA, Stein JL, Krostag AR, Nelson AM, Marken JS, Menon V, May RC, Yao Z, Kaykas A, Geschwind DH, Grimley JS. Nucleic Acids Res. 2015 Mar 11. pii: gkv164. 10.1093/nar/gkv164 PubMed 25765640
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