ER-mRFP
(Plasmid #62236)

• Purpose: Plasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.
• Depositing Lab: Erik Snapp
• Publication: Snapp et al Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6536-41. Epub 2006 Apr 14.

Backbone

• Vector backbone: clontech C1
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4800
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ER-mRFP
• Species: Synthetic
• Insert Size (bp): 801
• Promoter: cmv
• Tag / Fusion Protein:
  • mRFP1 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: tggtttagtgaaccgt
• 3′ sequencing primer: GTTCAGGGGGAGGTGTG

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Red localization marker for mammalian endoplasmic reticulum.