Proinsulin-NanoLuc in pLX304
(Plasmid #62057)

• Purpose: Luminescent reporter of insulin secretion
• Depositing Lab: David Altshuler
• Publication: Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi: 10.1016/j.cmet.2014.12.010.

Backbone

• Vector backbone: pLX304
• Backbone size w/o insert (bp): 9377
• Total vector size (bp): 8574
• Vector type: Mammalian Expression, Lentiviral, Luciferase
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Proinsulin-NanoLuc
• Species: M. musculus (mouse), Synthetic
• Insert Size (bp): 849
• Mutation: Inserted NanoLuc into C-peptide of mouse Ins2 coding sequence, with added PC2 sites flanking the luciferase
• Entrez Gene: Ins2 (a.k.a. Ins-2, InsII, Mody, Mody4)
• Promoter: CMV

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: V5

Resource Information

• Supplemental Documents:
  • Proinsulin-NanoLuc reporter
  • Proinsulin-NanoLuc reporter in pLX304 vector
• Addgene Notes:
  • Addgene Diagnostic Test
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry