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Addgene

B1979
(Plasmid #59181)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59181 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS(+)
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 1961
  • Total vector size (bp) 4503
  • Vector type
    Worm Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Blasticidin S resistance
  • Alt name
    BSD
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1593
  • Promoter rpl-28

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    B1979 was a gift from Craig Mello (Addgene plasmid # 59181 ; http://n2t.net/addgene:59181 ; RRID:Addgene_59181)

  • For your References section:

    A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis elegans. Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC. Genetics. 2014 May 30. pii: genetics.114.166389. 10.1534/genetics.114.166389 PubMed 24879462
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