pAAV-EF1a-FLEX-ArchT-GFP
(Plasmid
#58851)
-
PurposeAAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) manner
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 58851 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Want a viral vector made from this plasmid?
Make a packaging request and we'll get back to you.
Please log in to submit a packaging request.
-
SerotypeSelect serotype for details See details about
-
PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
-
How this works
- Place a request for a quantity of 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
- Addgene will quickly confirm that we can produce a high-quality prep for you.
- Track your request and place an order from within your account. Payment information must be added before we can begin processing your order.
- Receive your prep in 6–9 weeks after the MTA is approved by your organization.
- Learn more about our Packaged on Request Service.
Backbone
-
Vector backboneAAV-FLEX
-
Backbone manufacturerScott Sternson
- Backbone size w/o insert (bp) 6019
- Total vector size (bp) 7495
-
Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Growth instructionsStbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameArchT-GFP
-
Alt namearchaerhodopsin TP009
-
SpeciesHalorubrum sp. TP009
-
Insert Size (bp)1476
-
GenBank IDHM367071.1
- Promoter EF1a
-
Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BsrGI (not destroyed)
- 5′ sequencing primer cagggagctcaaaatgg
- 3′ sequencing primer cagcgtatccacatagcg (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Part of plasmid is sequenced by the depositing lab; the promoter, gene and fluorophore were sequenced. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-EF1a-FLEX-ArchT-GFP was a gift from Edward Boyden (Addgene plasmid # 58851 ; http://n2t.net/addgene:58851 ; RRID:Addgene_58851) -
For your References section:
A high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex. Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A, Baratta MV, Winkle J, Desimone R, Boyden ES. Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13. 10.3389/fnsys.2011.00018 PubMed 21811444