pAAV-EF1a-FLEX-ArchT-GFP
(Plasmid #58851)

• Purpose: AAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) manner
• Depositing Lab: Edward Boyden
• Publication: Han et al Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13.

Backbone

• Vector backbone: AAV-FLEX
• Backbone manufacturer: Scott Sternson
• Backbone size w/o insert (bp): 6019
• Total vector size (bp): 7495
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Stbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ArchT-GFP
• Alt name: archaerhodopsin TP009
• Species: Halorubrum sp. TP009
• Insert Size (bp): 1476
• GenBank ID: HM367071.1
• Promoter: EF1a
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: cagggagctcaaaatgg
• 3′ sequencing primer: cagcgtatccacatagcg

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Part of plasmid is sequenced by the depositing lab; the promoter, gene and fluorophore were sequenced. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.