pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation
(Plasmid
#58830)
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PurposeExpresses GFP-Tagged and mutated Osh2 PH domain in yeast
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58830 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS406
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Backbone manufacturerStratagene
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name2 x Osh2 PH domain
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Alt nameOsh2p
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SpeciesS. cerevisiae (budding yeast)
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Mutationchanged Lysine 307 to Glutamate and Arginine 309 to Glutamate on both Osh2 copies
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GenBank IDNM_001180078.1
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Entrez GeneOSH2 (a.k.a. YDL019C)
- Promoter PHO5
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Tags
/ Fusion Proteins
- GFP (N terminal on backbone)
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains two copies of Osh2 PH domain. Osh2 PH domain= Proline 256 - Proline 424
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation was a gift from Tim Levine (Addgene plasmid # 58830 ; http://n2t.net/addgene:58830 ; RRID:Addgene_58830) -
For your References section:
Multiple pools of phosphatidylinositol 4-phosphate detected using the pleckstrin homology domain of Osh2p. Roy A, Levine TP. J Biol Chem. 2004 Oct 22;279(43):44683-9. Epub 2004 Jul 22. 10.1074/jbc.M401583200 PubMed 15271978