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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22878 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSYFP2
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Alt namemVenus(L68V)
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SpeciesA. victoria
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Insert Size (bp)720
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site BsrGI (not destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSYFP2-C1 was a gift from Dorus Gadella (Addgene plasmid # 22878 ; http://n2t.net/addgene:22878 ; RRID:Addgene_22878) -
For your References section:
Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Foerster radius. Kremers GJ, Goedhart J, van Munster EB, Gadella TW. Biochemistry. 2006 May 30. 45(21):6570-80. 10.1021/bi0516273 PubMed 16716067