pSAG1::CAS9-U6::sgUPRT Citations (55)
Originally described in: Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9.Shen B, Brown KM, Lee TD, Sibley LD MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14. PubMed Journal
Articles Citing pSAG1::CAS9-U6::sgUPRT
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Genetic mapping reveals that sinefungin resistance in Toxoplasma gondii is controlled by a putative amino acid transporter locus that can be used as a negative selectable marker.
Behnke MS, Khan A, Sibley LD.
Eukaryot Cell. 2014 Dec 5. pii: EC.00229-14.
PubMed
Associated Plasmids |
| Rhoptry Proteins ROP5 and ROP18 Are Major Murine Virulence Factors in Genetically Divergent South American Strains of Toxoplasma gondii. Behnke MS, Khan A, Lauron EJ, Jimah JR, Wang Q, Tolia NH, Sibley LD. PLoS Genet. 2015 Aug 20;11(8):e1005434. doi: 10.1371/journal.pgen.1005434. eCollection 2015 Aug. PubMed |
Analysis of non-canonical calcium dependent protein kinases in Toxoplasma gondii by targeted gene deletion using CRISPR/Cas9.
Long S, Wang Q, Sibley LD.
Infect Immun. 2016 Jan 11. doi: 10.1128/IAI.01173-15
PubMed
Associated Plasmids |
| An improved method for introducing site-directed point mutation into the Toxoplasma gondii genome using CRISPR/Cas9. Sugi T, Kato K, Weiss LM. Parasitol Int. 2016 May 7. pii: S1383-5769(16)30109-X. doi: 10.1016/j.parint.2016.05.002. PubMed |
| The Past, Present, and Future of Genetic Manipulation in Toxoplasma gondii. Wang JL, Huang SY, Behnke MS, Chen K, Shen B, Zhu XQ. Trends Parasitol. 2016 Jul;32(7):542-53. doi: 10.1016/j.pt.2016.04.013. Epub 2016 May 12. PubMed |
| Functional characterization of X-prolyl aminopeptidase from Toxoplasma gondii. Yang M, Zheng J, Jia H, Song M. Parasitology. 2016 Sep;143(11):1443-9. doi: 10.1017/S0031182016000986. Epub 2016 May 25. PubMed |
| Analysis of the virulence determination mechanisms in a local Toxoplasma strain (T.gHB1) isolated from central China. Zhang W, Li L, Xia N, Zhou Y, Fang R, He L, Hu M, Shen B, Zhao J. Parasitol Res. 2016 May 25. PubMed |
| Characterization of aspartyl aminopeptidase from Toxoplasma gondii. Zheng J, Cheng Z, Jia H, Zheng Y. Sci Rep. 2016 Sep 28;6:34448. doi: 10.1038/srep34448. PubMed |
| Functional Characterization of Rhoptry Kinome in the Virulent Toxoplasma gondii RH Strain. Wang JL, Li TT, Elsheikha HM, Chen K, Zhu WN, Yue DM, Zhu XQ, Huang SY. Front Microbiol. 2017 Jan 24;8:84. doi: 10.3389/fmicb.2017.00084. eCollection 2017. PubMed |
| The moving junction protein RON4, although not critical, facilitates host cell invasion and stabilizes MJ members. Wang M, Cao S, DU N, Fu J, Li Z, Jia H, Song M. Parasitology. 2017 Jun 30:1-8. doi: 10.1017/S0031182017000968. PubMed |
| Translationally controlled tumor protein is required for the fast growth of Toxoplasma gondii and maintenance of its intracellular development. Zheng J, Chen Y, Li Z, Cao S, Zhang Z, Jia H. FASEB J. 2018 Feb;32(2):906-919. doi: 10.1096/fj.201700994R. Epub 2018 Jan 4. PubMed |
| Toxoplasma gondii Clp family protein: TgClpB1 plays a crucial role in thermotolerance. Cao S, Du N, Chen H, Pang Y, Zhang Z, Zheng J, Jia H. Oncotarget. 2017 Sep 18;8(49):86117-86129. doi: 10.18632/oncotarget.20989. eCollection 2017 Oct 17. PubMed |
| Conditional Knockdown of Proteins Using Auxin-inducible Degron (AID) Fusions in Toxoplasma gondii. Brown KM, Long S, Sibley LD. Bio Protoc. 2018 Feb 20;8(4). doi: 10.21769/BioProtoc.2728. PubMed |
| CRISPR-mediated Tagging with BirA Allows Proximity Labeling in Toxoplasma gondii. Long S, Brown KM, Sibley LD. Bio Protoc. 2018 Mar 20;8(6). doi: 10.21769/BioProtoc.2768. PubMed |
| Toxoplasma Chinese 1 Strain of WH3Deltarop16I/III /gra15II Genetic Background Contributes to Abnormal Pregnant Outcomes in Murine Model. Wang C, Cheng W, Yu Q, Xing T, Chen S, Liu L, Yu L, Du J, Luo Q, Shen J, Xu Y. Front Immunol. 2018 Jun 1;9:1222. doi: 10.3389/fimmu.2018.01222. eCollection 2018. PubMed |
| Characterization of Two EF-hand Domain-containing Proteins from Toxoplasma gondii. Chang L, Dykes EJ, Li J, Moreno SNJ, Hortua Triana MA. J Eukaryot Microbiol. 2019 Mar;66(2):343-353. doi: 10.1111/jeu.12675. Epub 2018 Aug 16. PubMed |
| A Lactate Fermentation Mutant of Toxoplasma Stimulates Protective Immunity Against Acute and Chronic Toxoplasmosis. Xia N, Zhou T, Liang X, Ye S, Zhao P, Yang J, Zhou Y, Zhao J, Shen B. Front Immunol. 2018 Aug 10;9:1814. doi: 10.3389/fimmu.2018.01814. eCollection 2018. PubMed |
| Elucidating the mitochondrial proteome of Toxoplasma gondii reveals the presence of a divergent cytochrome c oxidase. Seidi A, Muellner-Wong LS, Rajendran E, Tjhin ET, Dagley LF, Aw VY, Faou P, Webb AI, Tonkin CJ, van Dooren GG. Elife. 2018 Sep 11;7. pii: 38131. doi: 10.7554/eLife.38131. PubMed |
| Toxoplasma Effector TgIST Targets Host IDO1 to Antagonize the IFN-gamma-Induced Anti-parasitic Response in Human Cells. Bando H, Sakaguchi N, Lee Y, Pradipta A, Ma JS, Tanaka S, Lai DH, Liu J, Lun ZR, Nishikawa Y, Sasai M, Yamamoto M. Front Immunol. 2018 Sep 19;9:2073. doi: 10.3389/fimmu.2018.02073. eCollection 2018. PubMed |
| Inducible Nitric Oxide Synthase Is a Key Host Factor for Toxoplasma GRA15-Dependent Disruption of the Gamma Interferon-Induced Antiparasitic Human Response. Bando H, Lee Y, Sakaguchi N, Pradipta A, Ma JS, Tanaka S, Cai Y, Liu J, Shen J, Nishikawa Y, Sasai M, Yamamoto M. MBio. 2018 Oct 9;9(5). pii: mBio.01738-18. doi: 10.1128/mBio.01738-18. PubMed |
| Characterisation of the Toxoplasma gondii tyrosine transporter and its phosphorylation by the calcium-dependent protein kinase 3. Wallbank BA, Dominicus CS, Broncel M, Legrave N, MacRae JI, Staines HM, Treeck M. Mol Microbiol. 2018 Nov 6. doi: 10.1111/mmi.14156. PubMed |
| Micronemal protein 13 contributes to the optimal growth of Toxoplasma gondii under stress conditions. Ye S, Xia N, Zhao P, Yang J, Zhou Y, Shen B, Zhao J. Parasitol Res. 2019 Mar;118(3):935-944. doi: 10.1007/s00436-018-06197-3. Epub 2019 Jan 11. PubMed |
| Characterization of the ATP4 ion pump in Toxoplasma gondii. Lehane AM, Dennis ASM, Bray KO, Li D, Rajendran E, McCoy JM, McArthur HM, Winterberg M, Rahimi F, Tonkin CJ, Kirk K, van Dooren GG. J Biol Chem. 2019 Apr 5;294(14):5720-5734. doi: 10.1074/jbc.RA118.006706. Epub 2019 Feb 5. PubMed |
| The tyrosine transporter of Toxoplasma gondii is a member of the newly defined apicomplexan amino acid transporter (ApiAT) family. Parker KER, Fairweather SJ, Rajendran E, Blume M, McConville MJ, Broer S, Kirk K, van Dooren GG. PLoS Pathog. 2019 Feb 11;15(2):e1007577. doi: 10.1371/journal.ppat.1007577. eCollection 2019 Feb. PubMed |
| The Toxoplasma Vacuolar H(+)-ATPase Regulates Intracellular pH and Impacts the Maturation of Essential Secretory Proteins. Stasic AJ, Chasen NM, Dykes EJ, Vella SA, Asady B, Starai VJ, Moreno SNJ. Cell Rep. 2019 May 14;27(7):2132-2146.e7. doi: 10.1016/j.celrep.2019.04.038. PubMed |
| The Vacuolar Zinc Transporter TgZnT Protects Toxoplasma gondii from Zinc Toxicity. Chasen NM, Stasic AJ, Asady B, Coppens I, Moreno SNJ. mSphere. 2019 May 22;4(3). pii: 4/3/e00086-19. doi: 10.1128/mSphere.00086-19. PubMed |
| Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii. Hunt A, Russell MRG, Wagener J, Kent R, Carmeille R, Peddie CJ, Collinson L, Heaslip A, Ward GE, Treeck M. Elife. 2019 Oct 2;8. pii: 50598. doi: 10.7554/eLife.50598. PubMed |
| The Sec1/Munc18-like proteins TgSec1 and TgVps45 play pivotal roles in assembly of the pellicle and sub-pellicle network in Toxoplasma gondii. Cao S, Chen H, Liang X, Fu J, Wang S, Zheng J, Zhang Z, Pang Y, Wang J, Shen B, Jia H. Mol Microbiol. 2020 Jan;113(1):208-221. doi: 10.1111/mmi.14411. Epub 2019 Nov 19. PubMed |
| Profiling of myristoylation in Toxoplasma gondii reveals an N-myristoylated protein important for host cell penetration. Broncel M, Dominicus C, Vigetti L, Nofal SD, Bartlett EJ, Touquet B, Hunt A, Wallbank BA, Federico S, Matthews S, Young JC, Tate EW, Tardieux I, Treeck M. Elife. 2020 Jul 3;9. pii: 57861. doi: 10.7554/eLife.57861. PubMed |
| Divergent features of the coenzyme Q:cytochrome c oxidoreductase complex in Toxoplasma gondii parasites. Hayward JA, Rajendran E, Zwahlen SM, Faou P, van Dooren GG. PLoS Pathog. 2021 Feb 1;17(2):e1009211. doi: 10.1371/journal.ppat.1009211. eCollection 2021 Feb. PubMed |
| Identifying the major lactate transporter of Toxoplasma gondii tachyzoites. Zeng JM, Hapuarachchi SV, Shafik SH, Martin RE, Kirk K, van Dooren GG, Lehane AM. Sci Rep. 2021 Mar 24;11(1):6787. doi: 10.1038/s41598-021-86204-3. PubMed |
| Auranofin Resistance in Toxoplasma gondii Decreases the Accumulation of Reactive Oxygen Species but Does Not Target Parasite Thioredoxin Reductase. Ma CI, Tirtorahardjo JA, Jan S, Schweizer SS, Rosario SAC, Du Y, Zhang JJ, Morrissette NS, Andrade RM. Front Cell Infect Microbiol. 2021 Mar 19;11:618994. doi: 10.3389/fcimb.2021.618994. eCollection 2021. PubMed |
| The Dissection of SNAREs Reveals Key Factors for Vesicular Trafficking to the Endosome-like Compartment and Apicoplast via the Secretory System in Toxoplasma gondii. Cao S, Yang J, Fu J, Chen H, Jia H. mBio. 2021 Aug 31;12(4):e0138021. doi: 10.1128/mBio.01380-21. Epub 2021 Aug 3. PubMed |
| Toxoplasma gondii GRA28 Is Required for Placenta-Specific Induction of the Regulatory Chemokine CCL22 in Human and Mouse. Rudzki EN, Ander SE, Coombs RS, Alrubaye HS, Cabo LF, Blank ML, Gutierrez-Melo N, Dubey JP, Coyne CB, Boyle JP. mBio. 2021 Dec 21;12(6):e0159121. doi: 10.1128/mBio.01591-21. Epub 2021 Nov 16. PubMed |
| Toxoplasma bradyzoites exhibit physiological plasticity of calcium and energy stores controlling motility and egress. Fu Y, Brown KM, Jones NG, Moreno SN, Sibley LD. Elife. 2021 Dec 3;10. pii: 73011. doi: 10.7554/eLife.73011. PubMed |
| Toxoplasma gondii Rhoptry Protein 7 (ROP7) Interacts with NLRP3 and Promotes Inflammasome Hyperactivation in THP-1-Derived Macrophages. Zhu L, Qi W, Yang G, Yang Y, Wang Y, Zheng L, Fu Y, Cheng X. Cells. 2022 May 12;11(10). pii: cells11101630. doi: 10.3390/cells11101630. PubMed |
| Toxoplasma gondii CDPK3 Controls the Intracellular Proliferation of Parasites in Macrophages. Wu M, An R, Zhou N, Chen Y, Cai H, Yan Q, Wang R, Luo Q, Yu L, Chen L, Du J. Front Immunol. 2022 Jun 10;13:905142. doi: 10.3389/fimmu.2022.905142. eCollection 2022. PubMed |
| Cellular barcoding of protozoan pathogens reveals the within-host population dynamics of Toxoplasma gondii host colonization. Wincott CJ, Sritharan G, Benns HJ, May D, Gilabert-Carbajo C, Bunyan M, Fairweather AR, Alves E, Andrew I, Game L, Frickel EM, Tiengwe C, Ewald SE, Child MA. Cell Rep Methods. 2022 Aug 22;2(8):100274. doi: 10.1016/j.crmeth.2022.100274. eCollection 2022 Aug 22. PubMed |
| The first apicoplast tRNA thiouridylase plays a vital role in the growth of Toxoplasma gondii. Yang Y, Lin M, Chen X, Zhao X, Chen L, Zhao M, Yao C, Sheng K, Yang Y, Ma G, Du A. Front Cell Infect Microbiol. 2022 Aug 15;12:947039. doi: 10.3389/fcimb.2022.947039. eCollection 2022. PubMed |
| Apicoplast biogenesis mediated by ATG8 requires the ATG12-ATG5-ATG16L and SNAP29 complexes in Toxoplasma gondii. Fu J, Zhao L, Pang Y, Chen H, Yamamoto H, Chen Y, Li Z, Mizushima N, Jia H. Autophagy. 2023 Apr;19(4):1258-1276. doi: 10.1080/15548627.2022.2123639. Epub 2022 Sep 22. PubMed |
| CRISPR-based oligo recombineering prioritizes apicomplexan cysteines for drug discovery. Benns HJ, Storch M, Falco JA, Fisher FR, Tamaki F, Alves E, Wincott CJ, Milne R, Wiedemar N, Craven G, Baragana B, Wyllie S, Baum J, Baldwin GS, Weerapana E, Tate EW, Child MA. Nat Microbiol. 2022 Nov;7(11):1891-1905. doi: 10.1038/s41564-022-01249-y. Epub 2022 Oct 20. PubMed |
| Toxoplasma gondii AP2XII-2 Contributes to Transcriptional Repression for Sexual Commitment. Srivastava S, Holmes MJ, White MW, Sullivan WJ Jr. mSphere. 2023 Apr 20;8(2):e0060622. doi: 10.1128/msphere.00606-22. Epub 2023 Feb 14. PubMed |
| Late Embryogenesis Abundant Proteins Contribute to the Resistance of Toxoplasma gondii Oocysts against Environmental Stresses. Arranz-Solis D, Warschkau D, Fabian BT, Seeber F, Saeij JPJ. mBio. 2023 Apr 25;14(2):e0286822. doi: 10.1128/mbio.02868-22. Epub 2023 Feb 21. PubMed |
| The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments. Wan W, Dong H, Lai DH, Yang J, He K, Tang X, Liu Q, Hide G, Zhu XQ, Sibley LD, Lun ZR, Long S. Nat Commun. 2023 Feb 22;14(1):977. doi: 10.1038/s41467-023-36571-4. PubMed |
| An unconventional SNARE complex mediates exocytosis at the plasma membrane and vesicular fusion at the apical annuli in Toxoplasma gondii. Fu J, Zhao L, Yang J, Chen H, Cao S, Jia H. PLoS Pathog. 2023 Mar 27;19(3):e1011288. doi: 10.1371/journal.ppat.1011288. eCollection 2023 Mar. PubMed |
| Pantothenate biosynthesis in Toxoplasma gondii tachyzoites is not a drug target. Howieson VM, Zeng J, Kloehn J, Spry C, Marchetti C, Lunghi M, Varesio E, Soper A, Coyne AG, Abell C, van Dooren GG, Saliba KJ. Int J Parasitol Drugs Drug Resist. 2023 Aug;22:1-8. doi: 10.1016/j.ijpddr.2023.03.003. Epub 2023 Mar 15. PubMed |
| An essential role for an Fe-S cluster protein in the cytochrome c oxidase complex of Toxoplasma parasites. Leonard RA, Tian Y, Tan F, van Dooren GG, Hayward JA. PLoS Pathog. 2023 Jun 1;19(6):e1011430. doi: 10.1371/journal.ppat.1011430. eCollection 2023 Jun. PubMed |
| A screen of drug-like molecules identifies chemically diverse electron transport chain inhibitors in apicomplexan parasites. Hayward JA, Makota FV, Cihalova D, Leonard RA, Rajendran E, Zwahlen SM, Shuttleworth L, Wiedemann U, Spry C, Saliba KJ, Maier AG, van Dooren GG. PLoS Pathog. 2023 Jul 20;19(7):e1011517. doi: 10.1371/journal.ppat.1011517. eCollection 2023 Jul. PubMed |
| F-actin and myosin F control apicoplast elongation dynamics which drive apicoplast-centrosome association in Toxoplasma gondii. Devarakonda PM, Sarmiento V, Heaslip AT. mBio. 2023 Oct 31;14(5):e0164023. doi: 10.1128/mbio.01640-23. Epub 2023 Sep 21. PubMed |
| Susceptibility of Toxoplasma gondii to autophagy in human cells relies on multiple interacting parasite loci. Rinkenberger N, Rosenberg A, Radke JB, Bhushan J, Tomita T, Weiss LM, Sibley LD. mBio. 2024 Jan 16;15(1):e0259523. doi: 10.1128/mbio.02595-23. Epub 2023 Dec 14. PubMed |
| An apicoplast-localized deubiquitinase contributes to the cell growth and apicoplast homeostasis of Toxoplasma gondii. Xia J, Yang Y, Chen X, Song K, Ma G, Yang Y, Yao C, Du A. Vet Res. 2024 Jan 17;55(1):10. doi: 10.1186/s13567-023-01261-y. PubMed |
| Small RNAs from mitochondrial genome recombination sites are incorporated into T. gondii mitoribosomes. Tetzlaff S, Hillebrand A, Drakoulis N, Gluhic Z, Maschmann S, Lyko P, Wicke S, Schmitz-Linneweber C. Elife. 2024 Feb 16;13:e95407. doi: 10.7554/eLife.95407. PubMed |
| Myosin F controls actin organization and dynamics in Toxoplasma gondii. Kellermeier JA, Heaslip AT. Mol Biol Cell. 2024 Apr 1;35(4):ar57. doi: 10.1091/mbc.E23-12-0510. Epub 2024 Feb 28. PubMed |
| TBC9, an essential TBC-domain protein, regulates early vesicular transport and IMC formation in Toxoplasma gondii. Sun M, Tang T, He K, Long S. Commun Biol. 2024 May 18;7(1):596. doi: 10.1038/s42003-024-06310-6. PubMed |
| Toxoplasma gondii chitinase-like protein TgCLP1 regulates the parasite cyst burden. Bando H, Murata Y, Han Y, Sugi T, Fukuda Y, Bzik DJ, Fox BA, Kato K. Front Cell Infect Microbiol. 2024 May 17;14:1359888. doi: 10.3389/fcimb.2024.1359888. eCollection 2024. PubMed |
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