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Addgene

pMPRA3-CMV-Clover
(Plasmid #53540)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 53540 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMPRA3
  • Backbone manufacturer
    Broad Institute
  • Total vector size (bp) 5597
  • Vector type
    Mammalian Expression
  • Selectable markers
    Truncated H2Kk cell surface marker

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Clover GFP
  • Species
    Synthetic; Aequorea victoria
  • Insert Size (bp)
    717
  • Promoter CMV IE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The CMV-Clover cassette is based on Addgene Plasmid 40259 (pcDNA3-Clover) from the Michael Lin laboratory, but the physical DNA used to construct pMPRA-CMV-clover was synthesized de novo by Genscript Inc.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing detected an IS1 transposable element

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMPRA3-CMV-Clover was a gift from Tarjei Mikkelsen (Addgene plasmid # 53540 ; http://n2t.net/addgene:53540 ; RRID:Addgene_53540)

  • For your References section:

    Massively parallel reporter assays in cultured Mammalian cells. Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS. J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719. 10.3791/51719 PubMed 25177895
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