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Addgene

GSK3betaS9A  (1016)
(Plasmid #49492)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49492 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GSK3beta S9A
  • Alt name
    glycogen synthase kinase 3 beta
  • Alt name
    GSK3B
  • Alt name
    GSK3beta
  • Species
    H. sapiens (human)
  • Mutation
    S9A (constitutively active)
  • Entrez Gene
    GSK3B
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GSK3betaS9A  (1016) was a gift from Scott Friedman (Addgene plasmid # 49492 ; http://n2t.net/addgene:49492 ; RRID:Addgene_49492)

  • For your References section:

    GSK3beta phosphorylation of the KLF6 tumor suppressor promotes its transactivation of p21. Lang UE, Kocabayoglu P, Cheng GZ, Ghiassi-Nejad Z, Munoz U, Vetter D, Eckstein DA, Hannivoort RA, Walsh MJ, Friedman SL. Oncogene. 2012 Oct 22. doi: 10.1038/onc.2012.457. 10.1038/onc.2012.457 PubMed 23085750
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