pMPRA2
(Plasmid #49350)

• Purpose: (Empty Backbone) Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells
• Depositing Lab: Tarjei Mikkelsen
• Publication: Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719.

Backbone

• Vector backbone: pGL4
• Backbone manufacturer: Promega
• Backbone size (bp): 5862
• Modifications to backbone: Removed luc2 gene. Inserted cat-ccdB and PGK-H2Kk cassettes.
• Vector type: Mammalian Expression
• Selectable markers: Truncated H2Kk cell surface receptor

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Promega and Genscript (synthetic PGK-H2Kk insert)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the plasmid sequence contains an IS1 element, and the total size of the plasmid appears to be 7.5-8kb. This difference does not affect the function of the plasmid as described in the publication.