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Purpose(Empty Backbone) Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49349 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL4
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Backbone manufacturerPromega
- Backbone size (bp) 4238
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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A portion of this plasmid was derived from a plasmid made bycat-ccdB insert was PCA cloned from pLenti obtained from Addgene
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMPRA1 was a gift from Tarjei Mikkelsen (Addgene plasmid # 49349 ; http://n2t.net/addgene:49349 ; RRID:Addgene_49349) -
For your References section:
Massively parallel reporter assays in cultured Mammalian cells. Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS. J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719. 10.3791/51719 PubMed 25177895
Map uploaded by the depositor.
