pAc-sgRNA-Cas9 Citations (35)
Originally described in: Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9.Bassett AR, Tibbit C, Ponting CP, Liu JL Biol Open. 2013 Dec 10. pii: bio.20137120v1. doi: 10.1242/bio.20137120. PubMed Journal
Articles Citing pAc-sgRNA-Cas9
| Articles |
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Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9.
Bassett AR, Tibbit C, Ponting CP, Liu JL.
Biol Open. 2013 Dec 10. pii: bio.20137120v1. doi: 10.1242/bio.20137120.
PubMed
Associated Plasmids |
| Understanding functional miRNA-target interactions in vivo by site-specific genome engineering. Bassett AR, Azzam G, Wheatley L, Tibbit C, Rajakumar T, McGowan S, Stanger N, Ewels PA, Taylor S, Ponting CP, Liu JL, Sauka-Spengler T, Fulga TA. Nat Commun. 2014 Aug 19;5:4640. doi: 10.1038/ncomms5640. PubMed |
| Cas9-based genome editing in Drosophila. Housden BE, Lin S, Perrimon N. Methods Enzymol. 2014;546:415-39. doi: 10.1016/B978-0-12-801185-0.00019-2. PubMed |
A Genome-Wide CRISPR Library for High-Throughput Genetic Screening in Drosophila Cells.
Bassett AR, Kong L, Liu JL.
J Genet Genomics. 2015 Jun 20;42(6):301-9. doi: 10.1016/j.jgg.2015.03.011. Epub 2015 Apr 18.
PubMed
Associated Plasmids |
| Modifying an Insect Cell N-Glycan Processing Pathway Using CRISPR-Cas Technology. Mabashi-Asazuma H, Kuo CW, Khoo KH, Jarvis DL. ACS Chem Biol. 2015 Oct 16;10(10):2199-208. doi: 10.1021/acschembio.5b00340. Epub 2015 Aug 13. PubMed |
| RNA topoisomerase is prevalent in all domains of life and associates with polyribosomes in animals. Ahmad M, Xue Y, Lee SK, Martindale JL, Shen W, Li W, Zou S, Ciaramella M, Debat H, Nadal M, Leng F, Zhang H, Wang Q, Siaw GE, Niu H, Pommier Y, Gorospe M, Hsieh TS, Tse-Dinh YC, Xu D, Wang W. Nucleic Acids Res. 2016 Jun 1. pii: gkw508. PubMed |
| Chromatin binding of Gcn5 in Drosophila is largely mediated by CP190. Ali T, Kruger M, Bhuju S, Jarek M, Bartkuhn M, Renkawitz R. Nucleic Acids Res. 2016 Nov 29. pii: gkw1178. PubMed |
| Probing the canonicity of the Wnt/Wingless signaling pathway. Franz A, Shlyueva D, Brunner E, Stark A, Basler K. PLoS Genet. 2017 Apr 3;13(4):e1006700. doi: 10.1371/journal.pgen.1006700. eCollection 2017 Apr. PubMed |
| Aedes aegypti Piwi4 Is a Noncanonical PIWI Protein Involved in Antiviral Responses. Varjak M, Maringer K, Watson M, Sreenu VB, Fredericks AC, Pondeville E, Donald CL, Sterk J, Kean J, Vazeille M, Failloux AB, Kohl A, Schnettler E. mSphere. 2017 May 3;2(3). pii: e00144-17. doi: 10.1128/mSphere.00144-17. eCollection 2017 May-Jun. PubMed |
| Generation of genome-modified Drosophila cell lines using SwAP. Franz A, Brunner E, Basler K. Fly (Austin). 2017 Oct 2;11(4):303-311. doi: 10.1080/19336934.2017.1372068. Epub 2017 Aug 30. PubMed |
| Genomic Tagging of AGO1 Using CRISPR/Cas9-Mediated Homologous Recombination. Ghosh S, Liu JL. Methods Mol Biol. 2018;1680:217-235. doi: 10.1007/978-1-4939-7339-2_15. PubMed |
| In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering. Wu Q, Ferry QRV, Baeumler TA, Michaels YS, Vitsios DM, Habib O, Arnold R, Jiang X, Maio S, Steinkraus BR, Tapia M, Piazza P, Xu N, Hollander GA, Milne TA, Kim JS, Enright AJ, Bassett AR, Fulga TA. Nat Commun. 2017 Dec 13;8(1):2109. doi: 10.1038/s41467-017-00686-2. PubMed |
| ARE-mediated decay controls gene expression and cellular metabolism upon oxygen variations. de Toeuf B, Soin R, Nazih A, Dragojevic M, Jurenas D, Delacourt N, Vo Ngoc L, Garcia-Pino A, Kruys V, Gueydan C. Sci Rep. 2018 Mar 26;8(1):5211. doi: 10.1038/s41598-018-23551-8. PubMed |
| The Drosophila homologue of MEGF8 is essential for early development. Lloyd DL, Toegel M, Fulga TA, Wilkie AOM. Sci Rep. 2018 Jun 8;8(1):8790. doi: 10.1038/s41598-018-27076-y. PubMed |
| Sensitized genetic backgrounds reveal differential roles for EGF repeat xylosyltransferases in Drosophila Notch signaling. Pandey A, Li-Kroeger D, Sethi MK, Lee TV, Buettner FF, Bakker H, Jafar-Nejad H. Glycobiology. 2018 Nov 1;28(11):849-859. doi: 10.1093/glycob/cwy080. PubMed |
| Different mechanisms for selective transport of fatty acids using a single class of lipoprotein in Drosophila. Matsuo N, Nagao K, Suito T, Juni N, Kato U, Hara Y, Umeda M. J Lipid Res. 2019 Jul;60(7):1199-1211. doi: 10.1194/jlr.M090779. Epub 2019 May 13. PubMed |
| Time-Resolved Small RNA Sequencing Unravels the Molecular Principles of MicroRNA Homeostasis. Reichholf B, Herzog VA, Fasching N, Manzenreither RA, Sowemimo I, Ameres SL. Mol Cell. 2019 Aug 22;75(4):756-768.e7. doi: 10.1016/j.molcel.2019.06.018. Epub 2019 Jul 23. PubMed |
The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation.
Batki J, Schnabl J, Wang J, Handler D, Andreev VI, Stieger CE, Novatchkova M, Lampersberger L, Kauneckaite K, Xie W, Mechtler K, Patel DJ, Brennecke J.
Nat Struct Mol Biol. 2019 Aug;26(8):720-731. doi: 10.1038/s41594-019-0270-6. Epub 2019 Aug 5.
PubMed
Associated Plasmids |
| Programmable CRISPR interference for gene silencing using Cas13a in mosquitoes. Kulkarni A, Yu W, Moon AS, Pandey A, Hanley KA, Xu J. J Genomics. 2020 Mar 1;8:30-36. doi: 10.7150/jgen.43928. eCollection 2020. PubMed |
| Spt6 is a maintenance factor for centromeric CENP-A. Bobkov GOM, Huang A, van den Berg SJW, Mitra S, Anselm E, Lazou V, Schunter S, Feederle R, Imhof A, Lusser A, Jansen LET, Heun P. Nat Commun. 2020 Jun 10;11(1):2919. doi: 10.1038/s41467-020-16695-7. PubMed |
| Genome-wide identification of Aedes albopictus long noncoding RNAs and their association with dengue and Zika virus infection. Azlan A, Obeidat SM, Theva Das K, Yunus MA, Azzam G. PLoS Negl Trop Dis. 2021 Jan 22;15(1):e0008351. doi: 10.1371/journal.pntd.0008351. eCollection 2021 Jan. PubMed |
| Molecular principles of Piwi-mediated cotranscriptional silencing through the dimeric SFiNX complex. Schnabl J, Wang J, Hohmann U, Gehre M, Batki J, Andreev VI, Purkhauser K, Fasching N, Duchek P, Novatchkova M, Mechtler K, Plaschka C, Patel DJ, Brennecke J. Genes Dev. 2021 Mar 1;35(5-6):392-409. doi: 10.1101/gad.347989.120. Epub 2021 Feb 11. PubMed |
| Ago2 protects Drosophila siRNAs and microRNAs from target-directed degradation, even in the absence of 2'-O-methylation. Kingston ER, Bartel DP. RNA. 2021 Jun;27(6):710-724. doi: 10.1261/rna.078746.121. Epub 2021 Apr 14. PubMed |
| An Aedes aegypti-Derived Ago2 Knockout Cell Line to Investigate Arbovirus Infections. Scherer C, Knowles J, Sreenu VB, Fredericks AC, Fuss J, Maringer K, Fernandez-Sesma A, Merits A, Varjak M, Kohl A, Schnettler E. Viruses. 2021 Jun 3;13(6). pii: v13061066. doi: 10.3390/v13061066. PubMed |
| Cellular abundance shapes function in piRNA-guided genome defense. Genzor P, Konstantinidou P, Stoyko D, Manzourolajdad A, Marlin Andrews C, Elchert AR, Stathopoulos C, Haase AD. Genome Res. 2021 Nov;31(11):2058-2068. doi: 10.1101/gr.275478.121. Epub 2021 Oct 19. PubMed |
| Panoramix SUMOylation on chromatin connects the piRNA pathway to the cellular heterochromatin machinery. Andreev VI, Yu C, Wang J, Schnabl J, Tirian L, Gehre M, Handler D, Duchek P, Novatchkova M, Baumgartner L, Meixner K, Sienski G, Patel DJ, Brennecke J. Nat Struct Mol Biol. 2022 Feb;29(2):130-142. doi: 10.1038/s41594-022-00721-x. Epub 2022 Feb 16. PubMed |
| Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. Emtenani S, Martin ET, Gyoergy A, Bicher J, Genger JW, Kocher T, Akhmanova M, Guarda M, Roblek M, Bergthaler A, Hurd TR, Rangan P, Siekhaus DE. EMBO J. 2022 Jun 14;41(12):e109049. doi: 10.15252/embj.2021109049. Epub 2022 Mar 23. PubMed |
| Knockout of Sf-Caspase-1 generates apoptosis-resistant Sf9 cell lines: Implications for baculovirus expression. de Malmanche H, Marcellin E, Reid S. Biotechnol J. 2022 Jul;17(7):e2100532. doi: 10.1002/biot.202100532. Epub 2022 Apr 22. PubMed |
Functional editing of endogenous genes through rapid selection of cell pools (Rapid generation of endogenously tagged genes in Drosophila ovarian somatic sheath cells).
Meng Q, Stoyko D, Andrews CM, Konstantinidou P, Genzor P, O T, Elchert AR, Benner L, Sobti S, Katz EY, Haase AD.
Nucleic Acids Res. 2022 May 27. pii: 6594084. doi: 10.1093/nar/gkac448.
PubMed
Associated Plasmids |
| Developmental and housekeeping transcriptional programs in Drosophila require distinct chromatin remodelers. Hendy O, Serebreni L, Bergauer K, Muerdter F, Huber L, Nemcko F, Stark A. Mol Cell. 2022 Oct 6;82(19):3598-3612.e7. doi: 10.1016/j.molcel.2022.08.019. Epub 2022 Sep 15. PubMed |
Optimized In Vitro CRISPR/Cas9 Gene Editing Tool in the West Nile Virus Mosquito Vector, Culex quinquefasciatus.
Torres TZB, Prince BC, Robison A, Ruckert C.
Insects. 2022 Sep 19;13(9):856. doi: 10.3390/insects13090856.
PubMed
Associated Plasmids |
Endogenous transcripts direct microRNA degradation in Drosophila, and this targeted degradation is required for proper embryonic development.
Kingston ER, Blodgett LW, Bartel DP.
Mol Cell. 2022 Sep 15. pii: S1097-2765(22)00849-8. doi: 10.1016/j.molcel.2022.08.029.
PubMed
Associated Plasmids |
| Systematic identification and characterization of repressive domains in Drosophila transcription factors. Klaus L, de Almeida BP, Vlasova A, Nemcko F, Schleiffer A, Bergauer K, Hofbauer L, Rath M, Stark A. EMBO J. 2023 Feb 1;42(3):e112100. doi: 10.15252/embj.2022112100. Epub 2022 Dec 22. PubMed |
| Culex Mosquito Piwi4 Is Antiviral against Two Negative-Sense RNA Viruses. Walsh E, Torres TZB, Ruckert C. Viruses. 2022 Dec 10;14(12):2758. doi: 10.3390/v14122758. PubMed |
| Screening of Drosophila microRNA-degradation sequences reveals Argonaute1 mRNA's role in regulating miR-999. Sheng P, Li L, Li T, Wang Y, Hiers NM, Mejia JS, Sanchez JS, Zhou L, Xie M. Nat Commun. 2023 Apr 13;14(1):2108. doi: 10.1038/s41467-023-37819-9. PubMed |
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