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Addgene

pUb-Cas9-EGFP_cU6:6-sgRNA
(Plasmid #190597)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190597 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Self-assembly from various sources
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 10296
  • Vector type
    CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4101
  • Promoter Aedes aegypti polyubiquitin
  • Tag / Fusion Protein
    • EGFP - separated by T2A (C terminal on backbone)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    sgRNA
  • Insert Size (bp)
    21
  • Promoter Culex quinquefasciatus U6:6

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid was cloned from multiple sources: Ae. aegypti polyubiquitin promoter-driven Cas9 from Addgene plasmid #162163 the enhanced green fluorescent protein (eGFP) marker from Addgene plasmid #32425 Cx. quinquefasciatus RNA Pol III U6:6 promoter-driven sgRNA cloning site from Feng et al. 2021 (then adapted by our lab for BspQI cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUb-Cas9-EGFP_cU6:6-sgRNA was a gift from Claudia Rueckert (Addgene plasmid # 190597 ; http://n2t.net/addgene:190597 ; RRID:Addgene_190597)
  • For your References section:

    Optimized In Vitro CRISPR/Cas9 Gene Editing Tool in the West Nile Virus Mosquito Vector, Culex quinquefasciatus. Torres TZB, Prince BC, Robison A, Ruckert C. Insects. 2022 Sep 19;13(9):856. doi: 10.3390/insects13090856. 10.3390/insects13090856 PubMed 36135557