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Addgene

GFP-Mff
(Plasmid #49153)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49153 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGFP1-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4726
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mff
  • Alt name
    mitochondrial fission factor
  • Alt name
    Mff, isoform 1
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_020194
  • Entrez Gene
    MFF (a.k.a. C2orf33, EMPF2, GL004)
  • Promoter CMV
  • Tag / Fusion Protein
    • acGFP1 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Mff was a gift from Gia Voeltz (Addgene plasmid # 49153 ; http://n2t.net/addgene:49153 ; RRID:Addgene_49153)

  • For your References section:

    ER tubules mark sites of mitochondrial division. Friedman JR, Lackner LL, West M, DiBenedetto JR, Nunnari J, Voeltz GK. Science. 2011 Oct 21;334(6054):358-62. doi: 10.1126/science.1207385. Epub 2011 Sep 1. 10.1126/science.1207385 PubMed 21885730
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