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Addgene

BFP-Rab5
(Plasmid #49147)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTagBFP-C
  • Backbone manufacturer
    Evrogen
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab5
  • Alt name
    RAB5B, member RAS oncogene family
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_002868.3
  • Entrez Gene
    RAB5B
  • Promoter CMV
  • Tag / Fusion Protein
    • BFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer na
  • 3′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BFP-Rab5 was a gift from Gia Voeltz (Addgene plasmid # 49147 ; http://n2t.net/addgene:49147 ; RRID:Addgene_49147)

  • For your References section:

    Endoplasmic reticulum-endosome contact increases as endosomes traffic and mature. Friedman JR, Dibenedetto JR, West M, Rowland AA, Voeltz GK. Mol Biol Cell. 2013 Apr;24(7):1030-40. doi: 10.1091/mbc.E12-10-0733. Epub 2013 Feb 6. 10.1091/mbc.E12-10-0733 PubMed 23389631
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