pICXNHG3
(Plasmid #49021)

• Purpose: (Empty Backbone) FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site
• Depositing Labs: Raimund Dutzler, Eric Geertsma
• Publication: Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.

Backbone

• Vector backbone: pPICZ
• Backbone manufacturer: Invitrogen
• Backbone size (bp): 5764
• Modifications to backbone: modified for compatibility with FX cloning and added tags/fusion proteins
• Vector type: Yeast Expression
• Promoter: AOX1
• Selectable markers: Zeocin
• Tags / Fusion Proteins:
  • decaHis (10x His) (N terminal on backbone)
  • GFP (N terminal on backbone)
  • 3C protease site (PreScission site) (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Bleocin (Zeocin), 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: Low Copy

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: AOX1-fwd (5'-GACTGGTTCCAATTGACAAGC-3')
• 3′ sequencing primer: AOX1-rev (5'-GCAAATGGCATTCTGACATCC-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.