pRXTN
(Plasmid #47916)

• Purpose: (Empty Backbone) pRXTN is a modified version of pRX-tight Puro (part of the two plasmid Tet-ON system from Clontech) encoding NAT instead of PAC.
• Depositing Lab: James Iglehart
• Publication: Kochupurakkal et al PLoS One. 2013 Jul 4;8(7):e68509. doi: 10.1371/journal.pone.0068509. Print 2013.

Backbone

• Vector backbone: pRetro-X-Tight
• Backbone manufacturer: clontech
• Backbone size (bp): 6536
• Modifications to backbone: Puromycin resistance gene replaced with Nourseothricin resistance gene
• Vector type: Mammalian Expression, Retroviral
• Promoter: Tet
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN_5_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original NAT ORF was obtained from Prof Laura Knoll, University of Wisconsin-Madison. Sequence of the ORF was partially adapted to human codon usage.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry