pCMV4Neo-murine IL-17RA-HA
(Plasmid #46859)

• Purpose: expresses mouse IL-17RA
• Depositing Lab: Sarah Gaffen
• Publication: Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104(18):7506-11. Epub 2007 Apr 24.

Backbone

• Vector backbone: pCMV4-Neo
• Backbone size w/o insert (bp): 5750
• Total vector size (bp): 8400
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IL-17RA
• Species: M. musculus (mouse)
• Insert Size (bp): 2600
• Entrez Gene: Il17ra (a.k.a. AW538159, Cdw217, Il17r, VDw217)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Xba (upstream of 3xHA site) (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-PA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry