pCAG-Autobow
(Plasmid #45182)

• Depositing Lab: Joshua Sanes
• Publication: Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 2013 May 5.

Backbone

• Vector backbone: pCAG
• Total vector size (bp): 4800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pCAG-Autobow
• Insert Size (bp): 4800
• Promoter: CAG

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ACAAGTTTGTACAAAAAAGCAGGCT
• 3′ sequencing primer: ACCACTTTGTACAAGAAAGCTGGGT

Resource Information

• Supplemental Documents:
  • sequence/map
• A portion of this plasmid was derived from a plasmid made by: cloned by Kim Cohen and Dawen Cai
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry