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pST1374-NLS-flag-linker-Cas9
(Plasmid #44758)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44758 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pST1374 (Addgene #13426)
  • Backbone manufacturer
    pST1374-based custom vector
  • Backbone size w/o insert (bp) 5052
  • Total vector size (bp) 9318
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human codon optimized Cas9
  • Insert Size (bp)
    4266
  • Mutation
    codon-optimized
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • NLS (N terminal on insert)
    • 32 amino acid linker (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV-fwd
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The 32 amino acid linker sequence comes after the Flag tag and before the beginning of the Cas9 sequence. This 32 amino acid linker sequence is not depicted in the depositor's full plasmid sequence.

For more information on Huang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Huang/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pST1374-NLS-flag-linker-Cas9 was a gift from Xingxu Huang (Addgene plasmid # 44758 ; http://n2t.net/addgene:44758 ; RRID:Addgene_44758)

  • For your References section:

    Generation of gene-modified mice via Cas9/RNA-mediated gene targeting. Shen B, Zhang J, Wu H, Wang J, Ma K, Li Z, Zhang X, Zhang P, Huang X. Cell Res. 2013 Apr 2. doi: 10.1038/cr.2013.46. 10.1038/cr.2013.46 PubMed 23545779
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