pLV-mitoDsRed
(Plasmid #44386)

• Purpose: 3rd generation lentiviral vector
• Depositing Lab: Pantelis Tsoulfas
• Publication: Kitay et al Hum Mol Genet. 2013 Jan 24.

Backbone

• Vector backbone: pLV
• Backbone size w/o insert (bp): 6727
• Total vector size (bp): 7600
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Grow and plate on LB with low salt
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mitoDsRed
• Species: H. sapiens (human)
• Insert Size (bp): 880
• Promoter: CMV
• Tags / Fusion Proteins:
  • 61 aa targeting sequence of the P1 isoform F1F0-ATP synthase (N terminal on insert)
  • DsRed (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Carlos Moraes at the University of Miami
• Articles Citing this Plasmid:
  • 25 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Limitations of allotopic expression of mitochondrial genes in mammalian cells. Oca-Cossio J, Kenyon L, Hao H, Moraes CT.
Genetics. 2003 Oct;165(2):707-20. PMID: 14573482