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Addgene

pRG02
(Plasmid #44196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 5209
  • Vector type
    Worm Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neo
  • Alt name
    Aminoglycoside phosphotransferase
  • Alt name
    Aminoglycoside 3'-phosphotransferase
  • Alt name
    KanR
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2299
  • Promoter rps-27

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Rosina Giordano-Santini and Denis Dupuy from Denis Dupuy Lab (INSERM Bordeaux)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRG02 was a gift from Denis Dupuy (Addgene plasmid # 44196 ; http://n2t.net/addgene:44196 ; RRID:Addgene_44196)
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