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Addgene

pdestDD04Neo
(Plasmid #26386)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26386 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBS
  • Vector type
    Bacterial Expression, Worm Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB resistant bacteria ex: DB3.1 E. coli
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Species
    C. elegans (nematode)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pdestDD04Neo was a gift from Denis Dupuy (Addgene plasmid # 26386 ; http://n2t.net/addgene:26386 ; RRID:Addgene_26386)
  • For your References section:

    An antibiotic selection marker for nematode transgenesis. Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, Baillie D, Vidal M, Dupuy D. Nat Methods. 2010 Aug 22. ():. 10.1038/nmeth.1494 PubMed 20729841