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p3s-Cas9HC
(Plasmid #43945)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 43945 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 3200
  • Total vector size (bp) 7400
  • Modifications to backbone
    PvuII digestion and re-ligation to delete non-essential element in p3-Cas9HC.
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    4170
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • HA epitope (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information on Kim Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Kim/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3s-Cas9HC was a gift from Jin-Soo Kim (Addgene plasmid # 43945 ; http://n2t.net/addgene:43945 ; RRID:Addgene_43945)

  • For your References section:

    Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Cho SW, Kim S, Kim JM, Kim JS. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2507. 10.1038/nbt.2507 PubMed 23360966
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