pPV477
(Plasmid #42930)

• Depositing Lab: James Lok
• Publication: Lok lab Strongyloides stercoralis plasmids (unpublished)

Backbone

• Vector backbone: pDONR 221
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4762
• Total vector size (bp): 3417
• Vector type: Multi-site Gateway donor plasmid

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: attL1::egfp (870 bp)::attL2
• Alt name: EGFP
• Alt name: GFP
• Insert Size (bp): 870

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13_pUC_fwd
• 3′ sequencing primer: M13_rev

Resource Information

• Supplemental Documents:
  • pPV477.ape
• A portion of this plasmid was derived from a plasmid made by: egfp (870 bp) from pJA257 (Addgene plasmid #21510, Julie Ahringer lab)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry