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Addgene

GFP-EEA1 wt
(Plasmid #42307)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42307 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 4236
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EEA1
  • Alt name
    Early Endosome Antigen 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4236
  • Mutation
    none
  • GenBank ID
    NM_003566
  • Entrez Gene
    EEA1 (a.k.a. MST105, MSTP105, ZFYVE2)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-EEA1 wt was a gift from Silvia Corvera (Addgene plasmid # 42307 ; http://n2t.net/addgene:42307 ; RRID:Addgene_42307)

  • For your References section:

    The FYVE domain of early endosome antigen 1 is required for both phosphatidylinositol 3-phosphate and Rab5 binding. Critical role of this dual interaction for endosomal localization. Lawe DC, Patki V, Heller-Harrison R, Lambright D, Corvera S. J Biol Chem. 2000 Feb 4;275(5):3699-705. 10.1074/jbc.275.5.3699 PubMed 10652369
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